TATA binding protein TBP Antibody (1TBP18)

This antibody may be used in Western blot and electrophoretic mobility (gel shift) assays to detect mammalian TBP. It may also be used to immunoprecipitate a truncated TBP molecule containing only the N-terminal domain.

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein TBP and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.TPB is also known to interact with HIV1 Tat protein.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.

Showing Publications 1 - 9 of 9.

Publications using NB500-700	Applications	Species
Matsumura Y, Ito R, Yajima A et al. Spatiotemporal dynamics of SETD5-containing NCoR-HDAC3 complex determines enhancer activation for adipogenesis Nat Commun 2021-12-03 [PMID: 34857762]
Inagaki T, Iwasaki S, Matsumura Y et al. The FBXL10/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J. Biol. Chem. 2014-12-22 [PMID: 25533466] (Mouse)		Mouse
Lalevee S, Lapaire O, Buhler M. miR455 is linked to hypoxia signaling and is deregulated in preeclampsia. Cell Death Dis. 2014-09-04 [PMID: 25188518]
Sheldon Kathryn E, Shandilya Harish, Kepka-Lenhart Diane et al. Shaping the murine macrophage phenotype: IL-4 and cyclic AMP synergistically activate the arginase I promoter. J Immunol 2013-01-01 [PMID: 23913966] (WB, WB, Mouse)	WB, WB	Mouse
An CI, Ganio E, Hagiwara N. Trip12, a HECT domain E3 ubiquitin ligase, targets Sox6 for proteasomal degradation and affects fiber type-specific gene expression in muscle cells. Skelet Muscle 2013-05-10 [PMID: 23663701] (WB, Mouse)	WB	Mouse
Chuang CH, Wallace MD, Abratte C et al. Incremental genetic perturbations to MCM2-7 expression and subcellular distribution reveal exquisite sensitivity of mice to DNA replication stress. PLoS Genet. 2010-09-09 [PMID: 20838603] (WB, Mouse)	WB	Mouse
Wakabayashi K, Okamura M, Tsutsumi S et al. The peroxisome proliferator-activated receptor gamma/retinoid X receptor alpha heterodimer targets the histone modification enzyme PR-Set7/Setd8 gene and regulates adipogenesis through a positive feedback loop. Mol Cell Biol. 2009-07-01 [PMID: 19414603] (WB, Mouse)	WB	Mouse
Okamura M, Kudo H, Wakabayashi K-i et al. COUP-TFII acts downstream of Wnt/{beta}-catenin signal to silence PPAR{gamma} gene expression repress adipogenesis. PNAS;106(14):5819-5824. 2009-01-01 [PMID: 19307559]
Reagan-Shaw, S et al. RNA Interference-Mediated Depletion of Phosphoinositide 3-Kinase Activates Forkhead Box Class O Transcription Factors Induces Cell Cycle Arrest Apoptosis in Breast Carcinoma Cells. Cancer Res; 66: (2), 2006. 2006-01-01 [PMID: 16424042]

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Adam Causgrove	ELISA	Mouse	12/17/2010	View Summary Application ELISA Sample Tested Mouse Species Mouse
	4 reviewed by: DANISH SAYED	WB	Rat	09/23/2010	View Summary Application Western Blot Sample Tested Cultured Neonatal Myocytes, Sample Amount: 15-20ug of protein Species Rat

Array NB500-700

• TATA binding protein TBP Antibodies
• TATA binding protein TBP ELISA Kit
• TATA binding protein TBP Lysate
• TATA binding protein TBP Proteins