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Geminin Antibody (1B9) [DyLight 550]

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Product Details

Summary
Product Discontinued
View other related Geminin Primary Antibodies

Order Details


    • Catalog Number
      NB100-608R
    • Availability
      Product Discontinued

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Geminin Antibody (1B9) [DyLight 550] Summary

Immunogen
Full-length human geminin(amino acids 1-209).
Localization
Nuclear
Specificity
NB100-608 is specific for Geminin.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GMNN
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1-4 ug/ml
Application Notes
This antibody may be used for Western Blot where a band at ~30 kDa is recognized. We have seen better results using whole cell lysates than nuclear lysates, although nuclear lysates have been shown to give a band with an overnight exposure. May also be used for Immunoprecipitation.

Reactivity Notes

NB 100-608 recognizes human geminin protein.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
50mM sodium borate
Preservative
No Preservative
Concentration
1.7 mg/ml
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Geminin Antibody (1B9) [DyLight 550]

  • Gem
  • geminin
  • geminin, DNA replication inhibitor
  • RP3-369A17.3

Background

The DNA replication (origin) licensing system is necessary for duplicating the genome in each cell cycle. Geminin is a protein that prevents DNA replication by blocking the MCM complex incorporation into prereplication complex (pre-RC). It is absent during G1 phase and accumulates during S, G2, and M phases of mitosis. It is then degraded during the metaphase-anaphase trasition allowing replication to occur during subsequent cycles. Geminin has also been implicated in maintaining integrity of the male germline genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Geminin Antibody
(1B9) [DyLight 550]
NB100-608R
Geminin Antibody (1B9) [DyLight 550]
Species: Hu
Applications: WB, ICC/IF, IP

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Bioinformatics

Gene Symbol GMNN