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CCL2/MCP1 Antibody (5D3-F7)

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Product Details

Summary
Product Discontinued
View other related CCL2/MCP1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-78159
    • Availability
      Product Discontinued

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CCL2/MCP1 Antibody (5D3-F7) Summary

Immunogen
Recombinant human MCP-1
Specificity
Mouse Monoclonal to MCP-1 (5D3-F7)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CCL2
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. The purified 5D3-F7 has been tested by blocking fluorochrome conjugated 5D3-F7 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
Publications
Read Publication using NB100-78159.

Reactivity Notes

Expected Cross-Reactivity based on immunogen sequence: Cynomolgus, Rhesus.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CCL2/MCP1 Antibody (5D3-F7)

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL2/MCP1 Antibody
(5D3-F7)
NB100-78159
CCL2/MCP1 Antibody (5D3-F7)
Species: Hu, Pm
Applications: WB, ELISA, IHC, IP

Publications for CCL2/MCP1 Antibody (NB100-78159)(1)

We have publications tested in 1 confirmed species: Human.


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Reviews for CCL2/MCP1 Antibody (NB100-78159) (0)

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FAQs for CCL2/MCP1 Antibody (NB100-78159). (Showing 1 - 1 of 1 FAQ).

  1. I was looking for MCP1 antibody (5D3-F7) which has catalog number NB100-78159. I have surveyed few manuscript which mention the clone number (5D3-F7) but neither the company's name or the catalog number. I would like to whether all those reference indicate your product NB100-78159? We definitely want to purchase that antibody but make sure that is the right antibody.
    • I do not know whether those manuscripts mention our product because I am not familiar with those references. However, if it cites the same clone number for a particular target, it is more than likely the same antibody.

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Research Areas for CCL2/MCP1 Antibody (NB100-78159)

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Blogs on CCL2/MCP1.

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MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.

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Bioinformatics

Gene Symbol CCL2
Entrez