The mouse Insulin-like growth factor-binding protein 1 (IGFBP-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IGFBP-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IGFBP-1 concentration are added to these wells and allowed to complex with the bound IGFBP-1 antibody. A biotinylated mouse IGFBP-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGFBP-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IGFBP-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.



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