This recognizes human, mouse, and bovine HO-1. Other species have not been tested. It does not cross-react with HO-2.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide corresponding to aa 1-30 of human heme-oxygenase 1.
Marker:
Endoplasmic Reticulum Marker
Species Reactivity:
Human, Mouse and Bovine.
Applications:
Uses:
ELISA and Immunoblotting In IB a band of approx. 32 kDa is detected.
Dilutions:
ELISA 1ug/ml,
Western Blot 0.5-1ug/ml
Unit Size:
0.1 mg
Concentration:
Please see the vial label for concentration.
Packaging:
Storage:
Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:
PBS, pH 7.4
Preservative:
No Preservative
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Heme-oxygenase is an enzyme that catalyzes heme catabolism to yield biliverdin, iron, and carbon monoxide (CO). There are three isoforms of heme-oxygenase: HO-1, HO-2, and HO-3. HO-1 and HO-2 have been identified as the two major isoforms in mammals. HO-1, also known as heat shock protein 32 (Hsp32) is induced by most oxidative stress inducers, cytokines, inflammatory agents, and heat shock. HO-1 deficiency appears to cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Therefore, up-regulation of HO-1 is one of the major defense mechanisms against oxidative stress.