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Histone H2AX Antibody

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Product Details

Summary
Product Discontinued
View other related Histone H2AX Primary Antibodies

Order Details


    • Catalog Number
      NBP1-00871
    • Availability
      Product Discontinued

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Histone H2AX Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 100-150 of Human Histone H2A.X.
Specificity
X
Clonality
Polyclonal
Host
Rabbit
Gene
H2AX
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of Histone H2AX (S130) pAb in extracts from HT-29 cells.
Immunohistochemistry (IHC) analyzes of Histone H2AX (S130) pAb in paraffin-embedded human breast carcinoma tissue .
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse. May react with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H2AX Antibody

  • H2A.X
  • H2A/X
  • H2AFX
  • Histone H2AX

Background

H2AX is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2AX is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2AX at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2AX promotes DNA repair and maintains genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol H2AX
Entrez