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Histone H2AX Antibody

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Product Details

Summary
Product Discontinued
View other related Histone H2AX Primary Antibodies

Order Details


    • Catalog Number
      NB100-78355
    • Availability
      Product Discontinued

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Histone H2AX Antibody Summary

Immunogen
Recominant (partial), N-terminal
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AX
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Histone H2AX Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Histone H2AX Antibody

  • H2A.X
  • H2A/X
  • H2AFX
  • Histone H2AX

Background

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The Poly6133 antibody has been shown to be useful for Western blotting and immunofluorescence of human H2A.X.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Control Lysate(s)

Histone H2AX Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol H2AX
Entrez
Uniprot