FANCJ Antibody (NB100-416)
FANCJ Antibody
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Distributor Data...
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anti-MGC126523 antibody, anti-MGC126521 antibody, anti-FLJ90232 antibody, anti-ATP-dependent RNA helicase BRIP1 antibody, anti-EC 3.6.1 antibody, anti-Fanconi anemia group J protein antibody, anti-FANCJ protein antibody, anti-OF antibody, anti-BRIP 1 antibody, anti-BRCA 1 Interacting Protein 1 antibody, anti-BRAC 1 Associated C Terminal Helicase 1 antibody, anti-BACH1 BRIP1 antibody, anti-BACH 1 antibody, |
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FANCJ Antibody Summary: | | Gene: | BRIP1 |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | Specificity: | This is specific for BACH1/Brip1/FANCJ. |
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| Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional FANCJ Products FANCJ Antibody Details: | Immunogen: | A synthetic peptide within the extreme C-terminus of the human FANCJ/BACH1/Brip1 protein sequence.
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| Localization: | Nuclear |
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NB 100-416 recognizes human Brip1 / FANCJ |
| Uses: | This antibody recognizes a band at ~130 kDa, representing BRIP1/FANCJ. |
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| Dilutions: | immunoprecipitation ,
Western Blot 1:500,
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: BRIP1 (also called BACH1 /FANCJ) is a helicase that interacts with the BRCT domain of BRCA1 and has a role in BRCA1-dependent DNA repair and checkpoint. BRIP1 has recently been found to be defective in Fanconi anemia complementation group J. BRIP1/FANCJ has a function in the Fanconi anemia pathway that is independent of BRCA1 and downstream of FANCD2 activation. (1-4)
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Images (2) Reviews (2) Related Products (10) Exploring FANCJ Working with FANCJ
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with FANCJ
FANCJ Antibody Images (2) Brip 1 detected in cell lysates using NB 100-416. Lane 1: MCF-7 lysate, Lane 2: HeLa lysate, Lane 3: 293 lysate and Lane 4: SKOV3 lysate. |  Western Blot: FANCJ Antibody [NB100-416] - Western blot analysis on MCF7, HeLa, 293T and SKOV3 crude cell lysates using NB100-416. |
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Reviewed by:
Anonymous 3/5/2010
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Application:
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Western Blot
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Sample Tested:
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Human cell lines
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Sample Loaded:
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20ug total protein
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Lot:
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A
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Blocking Buffer:
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TBST with dry milk
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Concentration:
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10% dry milk
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Blocking Time:
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90min.
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Blocking Temperature:
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room temperature
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Dilution Ratio:
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1/2000
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Incubation Time:
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1 hour
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Incubation Temperature:
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room temperature
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Incubation Dilution Buffer:
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TBST
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Description:
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HRP conjugated donkey against rabbit antibody
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Dilution Ratio:
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1/5000
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Method:
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ECL
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Exposure Time:
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10 seconds
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Positive Control:
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Hela cell extract
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Negative Control:
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FANCJ deficient cell extract
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Did you observe bands?:
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True
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Non-specific Bands:
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not around specific band kDa
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Reviewed by:
Anonymous 3/4/2009
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Application:
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Western Blot
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Sample Tested:
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human fibroblasts
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Sample Loaded:
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20ug
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Lot:
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A
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Blocking Buffer:
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TBST
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Concentration:
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10
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Blocking Time:
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1 hour
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Blocking Temperature:
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25 Celsius
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Dilution Ratio:
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1/2000
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Incubation Time:
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1.5 hours
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Incubation Temperature:
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25 Celsius
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Incubation Dilution Buffer:
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TBST with 5% milk
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Description:
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Goat anti-rabbit IgG
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Dilution Ratio:
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1/5000
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Method:
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ECL
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Exposure Time:
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5 min.
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Did you observe bands?:
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True
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Specific Bands:
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170 kDa
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Publications (1)
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Ask a Scientist
Can't find an answer to your question about FANCJ? Ask us by using live
chat or by submitting your questions below:
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Jump to: Positive Controls, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- HeLa whole cell extract [NB 800-PC1], and Raji, 293, MCF7 and SKOV3 whole cell extracts.
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Primary Antibodies (9)RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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