ERK2 Antibody (NB100-78433)
ERK2 Antibody
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anti-Mitogen activated protein kinase 1 antibody, anti-p42 MAP Kinase antibody, anti-p41mapk antibody, anti-MAPK1 antibody, anti-Extracellular signal regulated kinase 2 antibody, |
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ERK2 Antibody Summary: | | Gene: | MAPK1 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ERK2 Products ERK2 Antibody Details: | Isotype: | IgG |
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| Immunogen: | Peptide mapping to the carboxy terminus of rat ERK2 |
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| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Dilutions: | Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot |
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| Unit Size: | 0.2 ml |
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| Concentration: | Please see the vial label for concentration. |
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| Positive Controls: | 2 Positive Controls |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | PBS [pH 7.2] |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: ERK2 is a 42 kD dual specificity kinase also known as mitogen activated protein kinase 1, extracellular signal regulated kinase 2, MAPK1, and p41mapk. The MAPK family includes several subgroups encompassing the ERK, JNK, and p38 kinases. ERK is phosphorylated in response to various extracellular signals inducing homodimerization and nuclear translocation where it phosphorylates target proteins regulating cell growth and survival. ERK2 interacts with a number of proteins including STAT5A, estrogen receptor alpha, ELK1, MEK1, protein kinase c epilson, RAF1, ribosomal S6 kinase, VAV1, STAT5b, Shc and many others. The Poly6242 antibody recognizes human, mouse, and rat ERK2 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
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Images (2) Reviews (0) Related Products (49) Exploring ERK2 Working with ERK2
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Jump to: Publications, Images, Ask a Question
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Working with ERK2
ERK2 Antibody Images (2) Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-ERK2 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system. |  Formalin-fixed paraffin-embedded human testis tissue was stained with Poly6242 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X. |
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Publications (2)ERK2 Antibody Background References:- Boulton, T.G., et al. 1991. Cell 65:663.
- Khokhlatchev, A.V., et al. 1998. Cell 93:605.
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ERK2 Lysate | NBL1-12865 | | WB | (1) | | | | Other Positive Controls- Ubiquitous cytoplasmic protein, translocates to nucleus upon activation.
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Lysates (2)Peptides and ProteinsPrimary Antibodies (42)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ERK2 Antibody (9E9) | NBP1-47846 | Hu, Rt | IHC-P, WB | (10) | | | | | ERK2 Antibody (6E5) | NBP1-47842 | Ca, Hu, Mk, Rt | IHC-P, WB | (8) | | | | | ERK2 Antibody (9H1) | NBP1-47845 | Ca, Hu, Mk, Rt | IHC-P, WB | (6) | | | | | ERK2 Antibody (4C2) | NBP1-47850 | Ca, Hu, Mk, Rt | IF, IHC-P, WB | (4) | | | | | ERK2 Antibody (6F8) | NBP1-47847 | Ca, Hu, Mk, Rt | FACS, IHC-P, WB | (4) | | | | | ... and 37 more. See All » |
RNAi (2)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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