ERK1 Antibody (NB100-1508)

ERK1 Antibody

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ERK1 Antibody Summary:
Species:Hu
Applications:PEP-ELISA
Clonality:Polyclonal
Gene:MAPK3
Purity:Immunogen affinity purified
Host:Goat
Specificity:No cross-reactivity expected with ERK2
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ERK1 Antibody Details:
Immunogen:Peptide with sequence GGEPRRTEGVGPC, from the N Terminus of the protein sequence according to NP_002737.1.
Species Reactivity:

Human

Applications:
Uses:This antibody is useful for Peptide ELISA and Western Blot. Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1).
Dilutions:Peptide ELISA 1:16000
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:Tris saline containing [pH7.3] with 0.5% BSA
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: MAPK3
Entrez5595 (Human)
Swiss ProtNP_002737.1. (Human)
 
Background:

ERK1 is ubiquitously expressed in various tissues and cells. It is activated by phosphorylation of neighbouring serine and threonine residues by MEK1 and MEK2 in response to diverse stimuli such as growth factors and mitogens.

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Working with ERK1

ERK1 Antibody Images (2)
HEK293 overexpressing MAPK3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-1508 in the right panel (vector-only transfection in first and fourth lanes).Western Blot: ERK1 Antibody [NB100-1508] - NB100-1508 (0.3ug/ml) staining of 293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Publications (1)

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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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RNAi (1)
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Secondary Antibodies
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ERK1 in Context


Research Areas related to ERK1 (8):
Novus Explorer for ERK1 gene

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