Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Peptide with sequence GGEPRRTEGVGPC, from the N Terminus of the protein sequence according to NP_002737.1.
Species Reactivity:
Human
Applications:
Uses:
This antibody is useful for Peptide ELISA and Western Blot. Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1).
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Tris saline containing [pH7.3] with 0.5% BSA
Preservative:
0.02% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
ERK1 is ubiquitously expressed in various tissues and cells. It is activated by phosphorylation of neighbouring serine and threonine residues by MEK1 and MEK2 in response to diverse stimuli such as growth factors and mitogens.
HEK293 overexpressing MAPK3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-1508 in the right panel (vector-only transfection in first and fourth lanes).
Western Blot: ERK1 Antibody [NB100-1508] - NB100-1508 (0.3ug/ml) staining of 293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.