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DAP1 Antibody (E59)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB110-56925
    • Availability
      Product Discontinued

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DAP1 Antibody (E59) Summary

Immunogen
A synthetic peptide corresponding to C-terminus of human DAP-1 was used as immunogen.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
DAP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-10000
Application Notes
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 11kDa.

Reactivity Notes

Reacts with: Human Does not react with Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
49% PBS, 0.05% BSA and 50% Glycerol
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for DAP1 Antibody (E59)

  • DAP1
  • DAP-1
  • death-associated protein 1
  • death-associated protein
  • MGC99796

Background

Death associated protein 1 (DAP-1) is a 15-kDa protein that functions as a positive mediator of cell death initiated by interferon (IFN-) gamma (1,2). DAP-1 is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). It is localized to the cytoplasm, but the detailed mechanism of its pro-apoptotic function is unclear. DAP-1 and DAP kinase, a unique, 160-kDa calmodulin-dependent serine/threonine kinase, are both unique candidates for positive mediators of programmed cell death induced by IFN-gamma (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for DAP1 Antibody (NB110-56925): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DAP1 Antibody (NB110-56925). (Showing 1 - 1 of 1 FAQ).

  1. I have a homology question. If you could get me the sequence of the immunogen that would be fantastic but, if not, a percent homology between the immunogen and the protein with GenBank Accession AFE84783 would work fine.
    • The peptide maps between amino acids 75 - 102, at the C-terminus of human DAP1 (UniProt: P51397). The exact immunogen shares 55% identity with GenBank Accession AFE84783, so I would not predict cross-reactivity. It is not guaranteed without testing.

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Bioinformatics

Gene Symbol DAP
Entrez
Uniprot