Jump to Information on Chromata ChIP Kit (NBP1-71709)
Chromata ChIP ChIP Kit Summary
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Tested Applications
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ChIP |
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Specificity
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This product contains all the necessary components to perform 25 Immunoprecipitation experiments including a positive control antibody (NB21-1023SS). |
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Kit Type
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ChIP Kit |
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Guarantee Plus
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All of our products are backed by our 100% guarantee to work for stated and predicted species and applications. |
Kit Components
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Components
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- RPL30 primer
- 10x Glycine
- IP Dilution Buffer
- H3 K4Me3 Positive Control Antibody
- Protein A/G Magnetic Beads
- IP Wash Buffer 1
- IP Wash Buffer 2
- IP Wash Buffer 3
- IP Wash Buffer 4
- IP Elution Buffer
- 5M NaCl
- Proteinase K
- Purification Columns
- Purification Column Tubes
- DNA Binding Buffer
- DNA Wash Buffer
- DNA Elution Buffer
- Human RPL30 Positive Control Primer Pair
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Species Reactivity
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Reviewed Species
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Read 2 rated 4.5 using NBP1-71709 in the following species: |
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Publications
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Read 1 using NBP1-71709 in the following species: |
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Applications/Dilutions
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Dilutions
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- Chromatin Immunoprecipitation
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Application Notes
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This ChromataChIP kit is a useful tool for Chromatin Immunoprecipitation. |
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Reviewed Applications
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Read 2 reviews rated 4.5 using NBP1-71709 in the following applications: |
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Publications
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Product PDFs
Reviews
Average Rating: 4.5
Recent Reviews
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Publications
Chromata ChIP Kit (NBP1-71709) has been mentioned in at least
4 publications.
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Controls & Support Products
Research Tools
Notes
Click
here for detailed information on the ChromataChIP kit
View the complete ChromatChIP Kit components and protocol
here.
Packaging, Storage & Formulations
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Storage
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Storage is content dependent. |
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Buffer
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Dependent on kit component. |
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Unit Size
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25 Immunoprecipitations |
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Concentration
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Concentration is not relevant for this product. Please see the protocols for proper use of this product. |
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Limitations
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This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt. |
Background
This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer.
Images for Chromata ChIP (5)
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Reviews for Chromata ChIP Kit (NBP1-71709) (2)
Read what people are saying who have used Chromata ChIP Kit (NBP1-71709).
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: Chromatin Immunoprecipitation.
Have you used Chromata ChIP Kit (NBP1-71709)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Submit a Review
Average Rating: 4.5  
(Based on 2 reviews)
| Sort 2 reviews for Chromata ChIP Kit (NBP1-71709) by: |
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| Images |
Ratings |
Applications |
Species |
Reviewed By |
Date |
Details |
 Click to Enlarge
Summary
- ApplicationChromatin Immunoprecipitation
- Sample TestedHEK293 and SH-SY5Y cells
- SpeciesHuman
- CommentsThis kit is extremely easy to use and really WORKS! I have struggled with kits in the past but the beads in this kit are superior to some other vendors and the positive control antibody and primers are brilliant. Also I have tried various reverse-crosslinking parameters but incubation at 95C for 15 minutes as stated in the protocol works perfectly!
Primary Antibody
- Dilution RatioH3K4me3 antibody provided in the kit.
Details
- Detection NotesRT-PCR using RPL30 positive control primers provided in the kit.
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Excellent |
ChIP |
Human |
Anonymous |
10/19/2012 |
More |
 Click to Enlarge
Summary
- ApplicationChromatin Immunoprecipitation
- Sample TestedHeLa nuclear extract
- Sample Pretreatment3,000,000 cells
- SpeciesHuman
- Lot031413
- CommentsI generally had a good experience with the kit.
Primary Antibody
- Dilution Ratio5ug, overnight 4C
Details
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Very Good |
ChIP |
Human |
Anonymous |
05/07/2013 |
More |
Publications for Chromata ChIP (4)
Read what people are writing who have used Chromata ChIP Kit (NBP1-71709).
We have 1 publication tested in 1 confirmed species: Human.
We have 3 tested in 1 confirmed application: ChIP.
Have you published using Chromata ChIP Kit (NBP1-71709)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Submit a Publication
| Publication |
Applications |
Species |
| Fishman J, Homon A, Lamsa E, et al. The Complete Chemical Synthesis of Histones H3 and H4 Containing Epigenetic Modifications and Their Use in Characterizing Arginine Methylated Histone Antibodies. Poster presented at ASCB. 21st Century Biochemicals, Marlborough, MA 01752, Novus Biologicals, LLC, 8100 Southpark Way, Littleton, CO 80120. |
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| Paul B. Noto, Yuri Bukhtiyarov, Meng Shi et al. Regulation of Sphingomyelin Phosphodiesterase, Acid-like 3A Gene (SMPDL3A) by Liver X Receptors. Molecular Pharmacology 2012 July 24 [PMID: 22810003] (ChIP) |
ChIP |
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| Noto PB, Bukhtiyarov Y, Meng S et al. Regulation of Sphingomyelin Phosphodiesterase, Acid-like 3A Gene (SMPDL3A) by Liver X Receptors Mol Pharmacol 2012 Jul 24 [PMID: 22810003] (ChIP) |
ChIP |
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| Sheela Rani CS, Soto-Pina A, Iacovitti L, Strong R. Evolutionary conservation of an atypical glucocorticoid-responsive element in the Human tyrosine hydroxylase gene. J Neurochem 2013 May 7 [PMID: 23647419] (ChIP, Human) |
ChIP |
Human |
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
ChIP Video Protocol |
ChIP Webinar |
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FAQs for Chromata ChIP (0)
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