Chromata ChIP ChIP Kit 25 Immunoprecipitations

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Chromatin Immunoprecipitation: Chromata ChIP ChIP Kit [NBP1-71709] - ChIP analysis of HeLa Nuclear Extracts. Image provided via product review by verified customer.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - ChIP analysis of HEK293 and SH-SY5Y cells. Image courtesy of an anonymous customer review.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - See notes for information on ChromataChIP Kit protocol information
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Ordering Information: NBP1-71709

  • Catalog Number
    NBP1-71709
  • Size(s)
    25 Immunoprecipitations
  • Availability
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Chromata ChIP ChIP Kit Summary

Tested Applications ChIP
Specificity
This product contains all the necessary components to perform 25 Immunoprecipitation experiments including a positive control antibody (NB21-1023SS).
Kit Type
ChIP Kit
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.

Kit Components

Species Reactivity


Reviewed Species Read 2 rated 4.5
using NBP1-71709 in the following species:

Publications Read Publications using NBP1-71709 in the following species:


Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
Application Notes
This ChromataChIP kit is a useful tool for Chromatin Immunoprecipitation.
Reviewed Applications
Read 2 Review rated 4.5
using
NBP1-71709 in the following application:

Publications
Read Publications using
NBP1-71709 in the following applications:

Contact Information

Product PDFs

Reviews

Average Rating: 4.5
Recent Reviews
ChIP Tested In Human
Reviewed On: 05/07/2013
Rated: 4

Chromata ChIP NBP1-71709
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Publications

Chromata ChIP Kit (NBP1-71709) has been mentioned in at least 4 publications.
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Notes

Click here for detailed information on the ChromataChIP kit View the complete ChromatChIP Kit components and protocol here.

Packaging, Storage & Formulations

Storage
Storage is content dependent.
Buffer
Dependent on kit component.
Unit Size
25 Immunoprecipitations
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Background

This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer.

Reviews for Chromata ChIP Kit (NBP1-71709) (2) 4.52

Read what people are saying who have used Chromata ChIP Kit (NBP1-71709).

We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1: Chromatin Immunoprecipitation.
Average Rating: 4.5
(Based on 2 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Chromatin Immunoprecipitation Chromata ChIP NBP1-71709
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Very Good
ChIP Human Anonymous 05/07/2013
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHeLa nuclear extract
Sample Pretreatment3,000,000 cells
SpeciesHuman
Lot031413
CommentsI generally had a good experience with the kit.

Primary Anitbody

Dilution Ratio5ug, overnight 4C

Details

Detection NotesqPCR
Chromatin Immunoprecipitation Chromata ChIP NBP1-71709
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Excellent
ChIP Human Anonymous 10/19/2012
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHEK293 and SH-SY5Y cells
SpeciesHuman
CommentsThis kit is extremely easy to use and really WORKS! I have struggled with kits in the past but the beads in this kit are superior to some other vendors and the positive control antibody and primers are brilliant. Also I have tried various reverse-crosslinking parameters but incubation at 95C for 15 minutes as stated in the protocol works perfectly!

Primary Anitbody

Dilution RatioH3K4me3 antibody provided in the kit.

Details

Detection NotesRT-PCR using RPL30 positive control primers provided in the kit.

Publications for Chromata ChIP Kit (NBP1-71709) (4)

Read what people are writing who have used Chromata ChIP Kit (NBP1-71709).

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 applications: ChIP.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar

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FAQs for Chromata ChIP (7)

  1. I received an inquiry about ChromataChIP Kit. http://www.novusbio.com/sites/default/files/images/chip_book_final_3.pdf Regarding phase 3(p.6), what size tube should be used? And what size tubes can be used for Magnetic tube separator rack (Cat# NBP1-72032)?
    • Generally this step is performed using 1.5mL tubes. The stand can hold eight 1.5-ml tubes or one 15-ml tube, and one 50-ml tube.
  2. I have one question about ChIP. In your Chromata ChIP kit, you add 25ul magnetic beads to the samples. Is there any way to normalize for differences in the amounts of magnetic beads in each sample? For example, it can happen that one sample got a bit more beads than another, is there some way to take this into account when you analyze your data?
    • As long as the vial/bottle of beads is vortexed so that the beads are fully resuspended in solution before taking the 25ul out, there will not be any significant variation in bead number. However, if you are taking multiple 25ul aliquots out, it is best to quickly revortex before every aliquot pipetting. For all of our internal testing, we typically see a CV of less than 2% for both intra- and interassay variation, which is very good.
  3. I am planning ChIP experiments using a rat cell line and also rat tissues. I am interested in your Chromata ChIP kit but I have some questions. The magnetic tube separator rack: is it possible to use a magnetic separator from Miltenyi Biotech (we already have one)? Is the positive control antibody (H3K4Me3) and primers (human RPL30) would work with rat samples? If no, could you provide a positive control working with the rat specie or give the primers sequences? Finally, what are the changes that have to be made to the protocol for ChIP assays starting from tissues? (Only an example using cells is given in the protocol)
    • 1. Yes, your magnetic rack will work just fine. 2. Unfortunately, our primers do not work with species other than human and we do not have any other primers available. You should be able to design your own to the same target in rat though and it should be just fine. 3. We are in the process of launching tissue specific protocols for use in ChIP. These are not available online yet but if you contact Gary Mack, our Lab Manager, he can assist you with this. His email is gary.mack@novusbio.com and he is aware that you will be in touch with him regarding this.
  4. Regarding the ChromataChIP Kit cat.#NBP1-71709: 1. What antibodies do you have? 2. Do you supply the qPCR primers for humans (positive and negative targets for each antibody)? 3. Do you supply the negative antibody? I like your guarantee, but hope never to hold you to it!
    • I am glad you are interested in our ChromataChIP Kits and would be happy to answer your questions. We have several ChIP grade epigenetics antibodies available and this link should take you to additional information about the kit itself and related products. I don't know if you saw this page first before inquiring further about the kit. Let me know if so and you still had additional questions and I would be happy to address those with the lab person who developed the kit. We are proud of our guarantee and of course prefer that no one would have to use it, but sometimes that is inevitable and as long as we can resolve any issues that arise are happy to do solid completely understand and know that is where your focus and attention will be. Let us know once you have more time on your hands if you are still interested in the lab visit and I can coordinate that with the lab.
  5. I wanted to know whether the H3K4me3 positive control antibody has cross reactivity with other species? I am using mouse tissue and would like to know whether this antibody will still work?
    • The positive control antibody in this kit is expected to cross-react with mouse, rat, c. elegans and most other mammals. It will work just fine for mouse.
  6. Do you know of anyone that has used this kit for plant tissues, preferably intact tissues? Also, do you have references where people have used your ChIP kit available?
    • We don't know of anyone using this kit on plants at this time. H3 is very well conserved, even in plants, as the immunogen to generate the antibody shares 100% similarity with tobacco, rice, maize and most other plants. This kit will almost certainly work with your plant. The main problem is that the primers almost certainly won't work on plants. Even one base pair difference can prevent the primers from working. Chances are there is some dissimilarity at the DNA level that doesn't effect the protein sequence because of the wobble rule. You can most likely use this kit on your plant, but you will need to design your own control primers.
  7. I was wondering if any of the Lightning-Link kit components contain phosphates, and whether you can tell me the concentration?
    • The Lightning-Link kits do contain phosphate. The final concentration will vary depending on the amount of antibody you add to the vial, but should always be less than 15mM.

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NBP1-71715
Western Blot: Protein A/G Magnetic Beads [NBP1-71715]Western Blot: Protein A/G Magnetic Beads [NBP1-71715] - Panel A: Rabbit serum; Panel B: Mouse serum. The IgG heavy chain bands were quantified by densitometry. The values for each set of duplicate bands were averaged and expressed as a percentage of the average for the Novus Biologicals Protein A/G Beads.


Applications IP, ChIP, WB

NB21-1063
Western Blot: Histone H3 [Sym-dimethyl Arg8] Antibody [NB21-1063] - WB analysis of Histone H3 R8me2s in C. elegans embryo lysate.Chromatin Immunoprecipitation: Histone H3 [Sym-dimethyl Arg8] Antibody [NB21-1063] - 2 ug of NB21-1063 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).Western Blot: Histone H3 [Sym-dimethyl Arg8] Antibody [NB21-1063] - WB analysis of Histone H3 R8me2s in synthetic full-length Histone H3 proteins.

Rabbit Polyclonal
Species Human, Mouse, C. elegans
Applications WB, ChIP, DB

1 Publication
NBP1-71709
Chromatin Immunoprecipitation: Chromata ChIP ChIP Kit [NBP1-71709] - ChIP analysis of HeLa Nuclear Extracts. Image provided via product review by verified customer.Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - ChIP analysis of HEK293 and SH-SY5Y cells. Image courtesy of an anonymous customer review.


Applications ChIP

     2 Reviews

4 Publications
NB21-2088
Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - Western blot analysis of HeLa histone prep lysate (A) and NIH 3T3 histone prep lysate (B) using Histone H4 [Monomethyl Lys20] antibody at 2 ug/ml.Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - Histone H4 K20me1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2088 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Rabbit Polyclonal
Species C. elegans, Mouse, Human
Applications ICC/IF, DB, ChIP...MORE

NB21-1002
Western Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - WB analysis of Histone H3 [Asym-dimethyl Arg2] in C. elegans embryo lysate.Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Histone H3 [Asym-dimethyl Arg2] antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Dot blot analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.

Rabbit Polyclonal
Species C. elegans, Mouse, Human
Applications ICC/IF, DB, ChIP...MORE

1 Publication

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