Human Breast Tissue Lysate (Adult Normal)

Clinical Tissue
Human Breast Normal Tissue Lysate

Tissue: Breast
Diagnosis:Infiltrating ductal carcinoma
Stage:IV TNM:T4N2Mx
Grade:2
Sex: Female
Age :45
Gross findings: Tumor size 8 cm. Cut section hard and yellow. Ipsilateral axillary lymph nodes examined: 8
Microscopic findings:
Metastasis to lymph nodes : Positive
Vascular infiltration: Positive
Tumor necrosis: Positive
Cellular differentiation: 2
Nuclear pleomorphism: 2
Mitotic activity: 1
Lymphocytic response: Positive

Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets. For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately.