Bcl2 Antibody (NB100-78342)
Bcl2 Antibody
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Distributor Data...
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anti-B-cell leukemia 2 antibody, |
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Bcl2 Antibody Summary: | | Gene: | BCL2 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Bcl2 Products Bcl2 Antibody Details: | Isotype: | IgG |
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| Immunogen: | Peptide-KLH, N-terminal |
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| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Dilutions: | immunofluorescence
Western Blot |
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| Unit Size: | 0.2 ml |
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| Concentration: | 0.5 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | phosphate-buffered solution, pH 7.2, with 0.1% gelatin and 50% glycerol |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist alpha=25 kD; beta=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. The Poly6119 antibody has been shown to be useful for Western blotting of the human, mouse, and rat Bcl-2 protein.
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Images (2) Reviews (0) Related Products (43) Exploring Bcl2 Working with Bcl2
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Jump to: Publications, Images, Ask a Question
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Working with Bcl2
Bcl2 Antibody Images (2) Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Bcl-2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody recognizes both the a and #223; Bcl-2 isoforms. |  Immunofluorescent: Bcl-2 Antibody [NB100-78342] - Immunofluorescent microscope analysis of Hela cells using anti-Bcl-2 polyclonal antibody, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI. |
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Publications (4)Bcl2 Antibody Background References:- Deng, X., et al., 2003. Blood. 102:3179.
- Huang, Z., et al., 2000. Oncogene 19:6627.
more... - Yang, E., et al., 1995. Cell 80:285.
- Tsujimoto, Y., et al., 1986 PNAS 83:5214.
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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
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Peptides and ProteinsPrimary Antibodies (35)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Bcl2 Antibody (E17) - Neuronal Apoptosis Inhibitor | NB110-55551 | Hu | FACS, ICC, IHC-P, IP, WB | (3) | (1) | | | | Bcl2 Antibody (8C8) - Neuronal Apoptosis Inhibitor | NB200-572 | Hu, Mk, Mu(-), Rt(-) | IHC-Fr, IHC-P, WB | (2) | (1) | | | | Bcl2 Antibody | NBP1-72199 | Bv, Ca, Hu, Mu, Rt | ELISA, IHC-P, IP, WB | (2) | (1) | | | | Bcl2 Antibody | NBP1-61508 | Hu, Mu, Rt | ELISA, IF, IHC-P, WB | (3) | | | | | Bcl2 Antibody | NB100-92142 | Hu, Mu, Rt | IF, IHC-P, WB | (3) | | | | | ... and 30 more. See All » |
RNAi (2)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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