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Bcl-2 Antibody (BCL/10C4)

 (4 Publications)
 (1 Review)
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Product Details

Summary
Product Discontinued
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    • Catalog Number
      NB100-78543
    • Availability
      Product Discontinued

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Bcl-2 Antibody (BCL/10C4) Summary

Immunogen
N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BCL2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
Application Notes
Immunoprecipitation and Immunofluorescence use reported in literature. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 30268882).
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-78543 in the following applications:
Publications
Read Publications using
NB100-78543 in the following applications:

  • IHC-P
    1 publication
  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Bcl-2 Antibody (BCL/10C4)

  • apoptosis regulator Bcl-2
  • B-cell CLL/lymphoma 2
  • Bcl2
  • Bcl-2

Background

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl2 family containing BH1-4 domains. Two reported isoforms exist alpha=25 kD; beta=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bcl-2 Antibody
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NB100-78543
Bcl-2 Antibody (BCL/10C4)
Species: Mu, Rt
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Publications for Bcl-2 Antibody (NB100-78543)(4)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC-P, WB.


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(1)
WB
(1)
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(2)
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Review for Bcl-2 Antibody (NB100-78543) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-78543:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Bcl-2 NB100-78543
Enlarge 		4
reviewed by:
Verified Customer
 WB Human 05/12/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotB133357

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bcl-2 Antibody (NB100-78543) (0)

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Research Areas for Bcl-2 Antibody (NB100-78543)

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Blogs on Bcl-2. Showing 1-10 of 11 blog posts - Show all blog posts.

Check out the latest blog posts on Bcl-2.

  Read full blog post.
Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.
Autophagy independent roles of the core ATG proteins
By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...  Read full blog post.
Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and ...  Read full blog post.
Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.
MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.
p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.
Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.
Beclin 1 - A Key Regulator of Autophagosome Formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.
BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.
Showing 1-10 of 11 blog posts - Show all blog posts.

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Recent Reviews

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Western Blot Bcl-2 NB100-78543
Enlarge
 Verified Customer
05/12/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol BCL2
Entrez
Uniprot