Bcl2 Antibody (NB100-78543)

Bcl2 Antibody (BCL/10C4)

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Bcl2 Antibody (BCL/10C4) Summary:
Species:Mu, Rt
Applications:FACS, ICC, IF, IP, WB
Clonality:Monoclonal
Gene:BCL2
Purity:Immunogen affinity purified
Host:Mouse
Specificity:Bcl-2 (BCL/10C4)
Publications:Antibody has at least 4 Background References.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Bcl2 Antibody (BCL/10C4) Details:
Clone:BCL/10C4
Isotype:IgG1 Kappa
Immunogen:N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Species Reactivity:

Mouse, Rat.

Applications:
Uses: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 mug/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Dilutions:Immunocytochemistry Flow Cytometry immunofluorescence immunoprecipitation Western Blot
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:This antibody is provided in phosphate-buffered solution, pH 7.2
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: BCL2
12043 (Mouse)
24224 (Rat)
 
Background:

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist alpha=25 kD; beta=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.

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Working with Bcl2

Bcl2 Antibody (BCL/10C4) Images (1)
Western Blot: Bcl-2 Antibody (BCL/10C4) [NB100-78543] - Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/10C4.
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Publications (4)

Bcl2 Antibody (BCL/10C4) Background References:

  1. Deng, X., et al., 2003. Blood. 102:3179.
  2. Huang, Z., et al., 2000. Oncogene 19:6627.

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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Bcl2 in Context


Research Areas related to Bcl2 (1):
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