The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
A synthetic peptide (FGYCNSSLNPVIYTIFNH) corresponding to amino acids 414-431 from human Adrenergic Receptor alpha 2a. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Species Reactivity:
Cross reacts with Human, Mouse, Rat and Rabbit.
Applications:
Uses:
This antibody is useful in Immunohistochemistry-Paraffin and Western Blot. Human Adrenergic Receptor alpha 2a has a predicted length of 450 amino acids and MW of 49 kDa.
Dilutions:
Immunohistochemistry-Paraffin 1.0-2.0 ug/ml, Western Blot 1.0 ug/ml
Unit Size:
0.1 mg
Concentration:
Lyoph
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4 Reconstitute in 0.1 ml of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Preservative:
0.05mg Thimerosal, 0.05mg Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq]. Sequence Note: The 5'-most in-frame translation initiation codon is selected for this RefSeq based on good conservation across mammalian species. A possible downstream start codon would result in a protein that is 15 aa shorter at the N-terminus. The literature, including PMIDs:2823383, 2568356, 1354394 and 1678390, assumes the use of the downstream start codon based on initial cloning reports, but there is no experimental evidence indicating which start codon is preferentially used in vivo. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by available transcript and conservation data. Two possible start codons are present in this update, with each having a weak Kozak signal. Due to leaky scanning by ribosomes, it is possible that some ribosomes may initiate translation from the upstream AUG while others start from the downstream AUG. Translation initiation from the downstream AUG would result in a protein that is 15 aa shorter at the N-terminus. The literature, including PMIDs 2823383, 2568356, 1354394 and 1678390, assumes the use of the downstream start codon based on initial cloning reports, but there is no experimental evidence indicating which start codon is preferentially used in vivo.
This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.