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Recombinant Human 14-3-3 eta Protein

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Product Details

Summary
Product Discontinued
View other related 14-3-3 eta Peptides and Proteins

Order Details


    • Catalog Number
      NBC1-18397
    • Availability
      Product Discontinued

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Recombinant Human 14-3-3 eta Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-246 of Human 14-3-3 eta

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
YWHAH
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
30.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris pH 8.0.
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human 14-3-3 eta Protein

  • 1433 eta
  • 14-3-3 eta
  • 14-3-3 protein eta
  • Protein AS1
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide
  • YWHA1
  • YWHA114-3-3 eta
  • YWHAH

Background

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, ', ', ', ', ', ' and ' that have been identified in mammals. The 14-3-3' (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3' interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3', fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol YWHAH