| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76701SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84576-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56421SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85691-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70702 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48854-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44404 | |||
| BSA Free | NBP2-76520-25ul | |||
![Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - In vitro nephrotoxicity assessment of kidney spheroids. Western blotting analysis of kidney injury markers in kidney spheroids after treatment with 20 uM cisplatin (CPT) or 50 uM cisplatin cyclosporin A (CSA). All data are shown as the means +/- s.e.m. *p < 0.05 compared with control by unpaired Student's t-test. Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-53855-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NBP1-76701-img0016.jpg "<b>Biological Strategies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - In vitro nephrotoxicity assessment of kidney spheroids. Western blotting analysis of kidney injury markers in kidney spheroids after treatment with 20 uM cisplatin (CPT) or 50 uM cisplatin cyclosporin A (CSA). All data are shown as the means +/- s.e.m. *p < 0.05 compared with control by unpaired Student's t-test. Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-53855-2) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Analysis of human uterus tissue using anti-TIM-1 antibody (3809) at 10 ug/ml.Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP)at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Immunohistochemistry-Paraffin-NBP1-76701-img0013.jpg)
![Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NBP1-76701-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), TIM-13811 (1 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), overnightincubation at 4C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NBP1-76701-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 ug of HeLa whole cell lysates per lane. Antibodies: 3809 (8 ug/mL), 1 hincubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NBP1-76701-img0015.jpg)
![Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 u of lysates per lane.Antibodies: TIM-1 (8 u/mL), overnight incubation at 4; C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76701_rabbit-polyclonal-tim-1-kim-1-havcr-antibody-2711202420171718.gif)
![Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Validation of TIM-1/KIM-1/HAVCR in Human Uterus Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1/KIM-1/HAVCR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.](http://images.novusbio.com/fullsize/nbp1-76701_rabbit-polyclonal-tim-1-kim-1-havcr-antibody-271120241937768.jpg)
![Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-western-blot-119202416527..gif "<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP1-76538-img0004.jpg)
![Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-NBP1-76538-img0003.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Flow-Cytometry-NBP1-76538-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Immunofluorescence of RHAMM/CD168 in human stomach tissue with RHAM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-2711202420243358.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining in human placenta and skeletal muscle tissues using anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human endometrium shows weak to moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0014.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0010.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0011.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-Immunohistochemistry-Paraffin-NBP1-84576-img0013.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0004.jpg "Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of A-549 cells, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0002.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0003.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Flow cytometry:1X10^6 Daudi cells (negative control,left) and K-562 cells were intracellularly-stained with Integrin alpha 5/CD49e Rabbit mAb or Rabbit IgG isotype control ,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517303123.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517352792.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251734518.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Confocal imaging of paraffin-embedded Human placenta using Integrin alpha 5/CD49e Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251734594.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517363728.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517344298.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Confocal imaging of paraffin-embedded Rat large intestine using Integrin alpha 5/CD49e Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251733528.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517363712.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Flow cytometry:1X10^6 Daudi cells (negative control,left) and U-87MG cells were intracellularly-stained with Integrin alpha 5/CD49e Rabbit mAb or Rabbit IgG isotype control ,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251733588.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517333885.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Western-Blot-NBP2-67883-img0009.jpg "Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2")
![Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunocytochemistry-Immunofluorescence-NBP2-67883-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0007.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of Jurkat cells with Integrin alpha 5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Flow-Cytometry-NBP2-67883-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunocytochemistry-Immunofluorescence-NBP2-67883-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0006.jpg)
![Western Blot: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Analysis of TIM-1/KIM-1/HAVCR in human brain lysate in the A) absence and B) presence of immunizing peptide, and C) mouse brain lysate using TIM-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Western-Blot-NB100-56421-img0003.jpg "Western Blot: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Analysis of TIM-1/KIM-1/HAVCR in human brain lysate in the A) absence and B) presence of immunizing peptide, and C) mouse brain lysate using TIM-1 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Caco-2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TIM-1/KIM-1/HAVCR Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56421-img0004.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Analysis in human liver and cerebral cortex tissues using NBP1-85691 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0026.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0021.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0025.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-85691-img0027.jpg)
![Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Simple Western lane view shows a specific band for CES1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Simple-Western-NBP1-85691-img0006.jpg)
![Simple Western: Carboxylesterase 1/CES1 Antibody [NBP1-85691] - Electropherogram image(s) of corresponding Simple Western lane view. CES1 antibody was used at 1:20 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Simple-Western-NBP1-85691-img0007.jpg)
![Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/DDR2-Antibody-Immunohistochemistry-Paraffin-NBP2-14926-img0001.jpg "Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: DDR2 Antibody [NBP2-14926] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/DDR2-Antibody-Western-Blot-NBP2-14926-img0004.jpg)
![Western Blot: DDR2 Antibody [NBP2-14926] - U87-MG whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDR2-Antibody-Western-Blot-NBP2-14926-img0005.jpg)
![Western Blot: DDR2 Antibody [NBP2-14926] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-91020242063328.jpg)
![DDR2 antibody [C2C3], C-term detects DDR2 protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DDR2 stained by DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:250.Red: Tau, an Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-immunocytochemistry-immunofluorescence-2611202518261315.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and DDR2 (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-western-blot-2611202518384019.jpg)
![Western Blot: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fc gamma RI/CD64.](http://images.novusbio.com/fullsize/Fc-gamma-RI-CD64-Antibody-OTI1A8-Western-Blot-NBP2-45625-img0005.jpg "Western Blot: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fc gamma RI/CD64.")
![Immunocytochemistry/Immunofluorescence: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Fc gamma RI/CD64 Antibody (1A8) [NBP2-45625] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Fc gamma RI/CD64.](http://images.novusbio.com/fullsize/Fc-gamma-RI-CD64-Antibody-OTI1A8-Immunocytochemistry-Immunofluorescence-NBP2-45625-img0001.jpg)
![Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Fc-gamma-RI-CD64-Antibody-OTI1A8-Immunohistochemistry-NBP2-45625-img0004.jpg)
![Flow Cytometry: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - analysis of living 293T cells transfected with FCGR1A overexpression plasmid .](http://images.novusbio.com/fullsize/Fc-gamma-RI-CD64-Antibody-OTI1A8-Flow-Cytometry-NBP2-45625-img0006.jpg)
![Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Fc-gamma-RI-CD64-Antibody-OTI1A8-Immunohistochemistry-NBP2-45625-img0002.jpg)
![Immunohistochemistry: Fc gamma RI/CD64 Antibody (OTI1A8) [NBP2-45625] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Fc-gamma-RI-CD64-Antibody-OTI1A8-Immunohistochemistry-NBP2-45625-img0003.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis in human liver and cerebral cortex tissues using NBP2-48854 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis in human liver and cerebral cortex tissues using NBP2-48854 antibody. Corresponding CES1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human cerebral cortex, liver, prostate and upper gastrointestinal using Anti-CES1 antibody NBP2-48854 (A) shows similar protein distribution across tissues to independent antibody NBP1-85691 (B).](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Analysis of human cell line Hep G2 shows positivity in endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48854-img0001.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0017.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0013.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0015.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0016.jpg)
![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP2-48854] - Staining of human small intestine shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Carboxylesterase-1-CES1-Antibody-Immunohistochemistry-Paraffin-NBP2-48854-img0018.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Western-Blot-NBP2-76520-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0005.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Western-Blot-NBP2-76520-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in human placenta and skeletal muscle tissues. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human placenta shows strong positivity in apical membranes of trophoblastic cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human liver shows strong positivity in liver sinusoids.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human prostate shows strong membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0007.jpg)
![Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDR2-Antibody-HL1107-Western-Blot-NBP3-13724-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDR2-Antibody-HL1107-Western-Blot-NBP3-13724-img0002.jpg)
![Western Blot: DDR2 Antibody (HL1107) [NBP3-13724] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-91020242011494.jpg)
![<b>Orthogonal Strategies Validation. </b> Mouse tissues.DDR2 stained by DDR2 antibody [HL1107] diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-317202591535..png)
![DDR2 antibody [HL1107] detects DDR2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.DDR2 stained by DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-2611202518404824.jpg)
![U87-MG whole cell and membrane extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-western-blot-2611202518475722.jpg)
![DDR2 antibody [HL1107] detects DDR2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.DDR2 stained by DDR2 antibody [HL1107] (NBP3-13724) diluted at 1:16000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13724_rabbit-ddr2-mab-hl1107-immunohistochemistry-paraffin-261120251843459.jpg)