CD44 Antibody (F10-44-2)

Immunocytochemistry/Immunofluorescence: CD44 Antibody (F10-44-2) [NBP1-28385] - Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT Streptavidin-TXRD and DAPI.

Immunohistochemistry-Paraffin: CD44 Antibody (F10-44-2) [NBP1-28385] - Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB and Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-AP and Goat Anti-Mouse IgG2b, Human ads-HRP Red AP, DAB, and mounted with Fluoromount-G® .

Immunocytochemistry/Immunofluorescence: CD44 Antibody (F10-44-2) [NBP1-28385] - Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB followed by Donkey Anti-Mouse IgG(H+L), Human SP ads- and DAPI.

Immunohistochemistry-Paraffin: CD44 Antibody (F10-44-2) [NBP1-28385] - Human gastric cancer tissue was stained with Mouse Anti-Human CD44-UNLBfollowed by Goat Anti-Mouse IgG2a, Human ads-AP Red AP, hematoxylin, and mounted with Fluoromount-G®.

Product Discontinued

CD44 Antibody (F10-44-2) Summary

• Additional Information: Clone F10-44-2 was used by HLDA to establish CD designation.
• Immunogen: Purified T cells from human lymph nodes.
• Marker: Cell Membrane Marker
• Specificity: Human/Ma's Night Monkey CD44.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 1:10 - 1:1000
  • Functional Assay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD44 Antibody (F10-44-2)

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44, also known as phagocytic glycoprotein-1 (Pgp-1) and the receptor for hyaluronate, exists as a large number of different isoforms resulting from alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells and erythrocytes is a glycosylated type I transmembrane protein. Other variable isoforms containing glycosaminoglycans have molecular weights ranging from 110 to 250 kDa and are widely expressed on hematopoietic and non-hematopoietic cells. CD44 contributes to the adhesion of leukocytes to endothelial cells, stromal cells and the extracellular matrix. It also plays a functional role in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation. (1-6)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD44