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| Application Notes | Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents . Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | 1X SDS-PAGE Sample Buffer [62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8], 10% (v/v) Glycerol |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Type | Tissue |
| Life Stage | Adult |
| Tissue | Digestive/Waste |
| Protein State | Native |
| Tissue Condition | Normal |
| Subcellular Fraction | Whole |
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