Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Synthetic peptide corresponing to the residues surrounding Methionine 270 of human NFkappaB-p65
Species Reactivity:
Human, Mouse, Rat
Applications:
Uses:
This antibody is useful for Western Blot, Immunohistochemistry and Immunoprecipitation. Western blot analysis of NFkappaB-p65 (M270) antibody in extracts from HeLa cells. Immunohistochemistry analyzes of NFkappaB-p65 (M270) antibody in paraffin-embedded human breast carcinoma tissue.
Dilutions:
Immunohistochemistry-Paraffin 1:50-1:200, immunoprecipitation 1:50-1:200, Western Blot 1:500-1:1000
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Phosphate buffered saline (PBS), approx. pH 7.2.
Preservative:
15mM Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in
many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity.