Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The epitope recognized by this antibody maps to a region between residue 248 and 298 of human cyclin-dependent kinase 2 using the numbering given in entry NP_001789.2 (GeneID 1017).
Species Reactivity:
Human
Applications:
Uses:
This antibody may be used for western blot and immunoprecipitation.
Dilutions:
Western Blot 1:2000-1:10000,
immunoprecipitation 2-5 mcg/mg lysate,
Unit Size:
0.1 ml
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]
Western Blot and Immunoprecipitation: CDK2 Antibody [NB100-95002] - Detection of Human CDK2 on HeLa whole cell lysate using NB100-95002. CDK2 was also immunoprecipitated, albeit less efficiently, by another rabbit anti-CDK2 antibody.