Cdk2 Antibody (NB100-81843)

Cdk2 [p Thr160] Antibody

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Cdk2 [p Thr160] Antibody Summary:
Species:Hu, Mu, Rt
Applications:ELISA, IHC-P, WB
Clonality:Polyclonal
Gene:CDK2
Purity:Immunogen affinity purified
Host:Rabbit
Specificity:CDK2 (specific to Phospho-Thr160) detects endogenous levels of CDK2 only when phosphorylated at threonine 160.
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Cdk2 [p Thr160] Antibody Details:
Isotype:IgG
Immunogen:The antiserum was produced against synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of threonine 160 (T-Y-TP-H-E).
Modification:p Thr160
Species Reactivity:

Human, Mouse, Rat

Applications:
Uses:ELISA, Western Blot and Immunohistochemistry-Paraffin
Dilutions:ELISA 1:4000, Immunohistochemistry-Paraffin 1:50-1:100, Western Blot 1:500-1:1000
Unit Size:0.05 mg
Concentration:1.0 mg/ml
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CDK2
Entrez1017 (Human)
12566 (Mouse)
362817 (Rat)
Swiss ProtP24941 (Human)
 
Background:

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

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Working with Cdk2

Cdk2 [p Thr160] Antibody Images (2)
Western Blot: CDK2 Phosphospecific Antibody [NB100-81843] - Western blot analysis of extracts from A2780 cells (Lane 1 and 2) and MDA-MB-435 cells (Lane 3), using CDK2 (phospho-Thr160) antibodyImmunohistochemistry: CDK2 Phosphospecific Antibody [NB100-81843] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using CDK2 (phospho-Thr160) antibody
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Publications (1)

Cdk2 [p Thr160] Antibody Background References:

  1. Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6 Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53 Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56 Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14

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Jump to: Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Cdk2 in Context


Research Areas related to Cdk2 (1):
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