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Cdk2 Antibody (NB100-1694)

Cdk2 Antibody

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Cdk2 Antibody Summary:
Species:Hu, Rt
Applications:IHC, IHC-P, WB
Clonality:Polyclonal
Gene:CDK2
Purity:Whole antisera
Host:Rabbit
Specificity:No reaction was observed against other related cyclin dependent kinases.
Publications:Antibody has at least 3 Background References.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Cdk2 Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide (Human) conjugated to KLH.
Localization:Cytoplasmic and Nuclear
Species Reactivity:

Cross-reacts with Human and Rat. Not yet tested in other species.

Applications:
Uses:This antibody is useful for Western Blot and Immunohistochemistry-Paraffin
Dilutions:Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot 1:400
Unit Size:0.1 ml
Concentration:This product is unpurified. Concentration is not relevant.
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:Whole antiserum, sterile filtered.
Preservative:0.01% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CDK2
Entrez1017 (Human)
362817 (Rat)
 
Images (2)
  • Western Blot: Cdk2 Antibody [NB100-1694] - Immunoblotting. Rabbit anti-cdk2 was used at a 1:200 dilution to detect cdk-2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 ug of lysate was separated on a 15% SDS-PAGE gel. Cdk-2 is indicated by an arrowhead as a 32-33 kDa band. Note that multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Primary antibody was reacted at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
  • Immunohistochemistry: Cdk2 Antibody [NB100-1694] - Anti-CDK2 antibody was diluted 1:500 to detect CDK2 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pretreatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

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Working with Cdk2

Cdk2 Antibody Images (2)
Western Blot: Cdk2 Antibody [NB100-1694] - Immunoblotting. Rabbit anti-cdk2 was used at a 1:200 dilution to detect cdk-2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 ug of lysate was separated on a 15% SDS-PAGE gel. Cdk-2 is indicated by an arrowhead as a 32-33 kDa band. Note that multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Primary antibody was reacted at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.Immunohistochemistry: Cdk2 Antibody [NB100-1694] - Anti-CDK2 antibody was diluted 1:500 to detect CDK2 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pretreatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Publications (3)

Cdk2 Antibody Background References:

  1. Tsai LH et al. Isolation of the human cdk2 gene that encodes the cyclin A- adenovirus E1A-associated p33 kinase. Nature 353:174-7 (1991).
  2. Giordano A et al. Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A. Science 253:1271-5 (1991).

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