| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB120-7111 | |||
| Pre-adsorbed | NB7584 | |||
| Pre-adsorbed | NBP1-72650 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75427 | |||
| Biotin | NBP1-75428 | |||
| DyLight 350 | NBP1-75666 | |||
| DyLight 488 | NBP1-75432 | |||
| DyLight 594 | NBP1-75668 | |||
| DyLight 633 | NBP1-75669 | |||
| FITC | NBP1-75429 | |||
| HRP | NBP1-75430 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-75352 | |||
| HRP | NBP1-75353 | |||
![Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Image from the HRP version of this antibody.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6710-img0012.jpg "Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT. Block for30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Image from the HRP version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Immunocytochemistry/Immunofluorescence: Sheep anti-Mouse IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72924]](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB120-6710-img0009.jpg)
![Western Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NB120-6710-img0004.jpg)
![Dot Blot: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Mouse IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Dot-Blot-NB120-6710-img0001.jpg)
![Fluorophore-linked immunosorbent assay: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - Fluorophore-linked immunosorbent assay: Sheep anti-Mouse IgG, H/L Chains Antibody [DyLight 405] [NBP1-72929]](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-6710-img0002.jpg)
![ELISA: Sheep anti-Mouse IgG (H+L) Secondary Antibody [NB120-6710] - ELISA results of purified Sheep anti-Mouse IgG (H+L) Secondary antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Dky-anti-Sheep HRP conjugate, and TMB substrate.](http://images.novusbio.com/fullsize/Sheep-anti-Mouse-IgG-H+L-Secondary-Antibody-ELISA-NB120-6710-img0014.jpg)
![Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72745-img0008.jpg "Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.")
![Immunocytochemistry/Immunofluorescence: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - Immunocytochemistry/Immunofluorescence: Donkey anti-Sheep IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-73002] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72745-img0003.jpg)
![ELISA: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - ELISA results of purified Polyclonal Donkey anti-Sheep IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed) tested against Sheep IgG. Each well was coated in duplicate with 1.0ug of antigen. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer. Using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72745-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72745-img0009.jpg)
![ELISA: Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72745] - ELISA Results of Donkey anti-Sheep IgG (H+L) Secondary antibody (Pre-adsorbed) tested against purified Sheep IgG Mx8. Each well was coated in duplicate with 1.0 ug of Sheep IgG [Red Line], Chicken IgG [Green Line], Guinea Porcine IgG [Blue Line], Hamster IgG [Purple Line], Equine IgG, Human IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution of Sheep IgG MX8 is 1:49,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72745-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - Immunocytochemistry/Immunofluorescence: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72996] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Sheep-IgG-H+L-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72704-img0004.jpg "Immunocytochemistry/Immunofluorescence: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - Immunocytochemistry/Immunofluorescence: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72996] Image from the DyLight 488 version of this antibody.")
![Dot Blot: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - Sheep IgG. Load: 200 ug, 66.7 ug, 22.2 ug, 7.41 ug or 2.47 ug as indicated. Primary antibody: N/A. Secondary antibody: Alkaline Phosphatase Conjugated Rabbit anti-Sheep IgG Antibody. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 1 hour.](http://images.novusbio.com/fullsize/Rabbit-anti-Sheep-IgG-H+L-Secondary-Antibody-Dot-Blot-NBP1-72704-img0001.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Sheep-IgG-H+L-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72704-img0007.jpg)
![ELISA: Rabbit anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72704] - ELISA results of purified Rabbit anti-Sheep IgG (H+L) Secondary Antibody [Unconjugated] tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 ug of Sheep IgG (purple line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed),, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Sheep-IgG-H+L-Secondary-Antibody-ELISA-NBP1-72704-img0008.jpg)
![Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72699] - Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG. Other band(s): None. Image using the Texas Red format of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-72699-img0003.jpg "Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72699] - Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG. Other band(s): None. Image using the Texas Red format of this antibody.")
![Dot Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72699] - Sheep IgG Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-Dot-Blot-NBP1-72699-img0001.jpg)
![ELISA: Donkey anti-Sheep IgG (H+L) Secondary Antibody [NBP1-72699] - ELISA results of purified ELISA of Donkey anti-Sheep IgG (H+L) Secondary Antibody [Unconjugated] tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 ug of Sheep IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Gt-a-Donkey IgG HRP conjugate 1:10000, and TMB substrate.](http://images.novusbio.com/fullsize/Donkey-anti-Sheep-IgG-H+L-Secondary-Antibody-ELISA-NBP1-72699-img0005.jpg)
![ELISA: Rabbit anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72744] - ELISA Results of Rabbit anti-Sheep IgG (H+L) Secondary antibody (Pre-adsorbed) (Min X Human Serum Proteins) tested against purified Sheep IgG MX Hu. Each well was coated in duplicate with 1.0 ug of Sheep IgG [Green Line] and Human IgG [Blue Line]. The working dilution of Sheep IgG MX Hu is 1:71,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Sheep-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72744-img0001.jpg "ELISA: Rabbit anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72744] - ELISA Results of Rabbit anti-Sheep IgG (H+L) Secondary antibody (Pre-adsorbed) (Min X Human Serum Proteins) tested against purified Sheep IgG MX Hu. Each well was coated in duplicate with 1.0 ug of Sheep IgG [Green Line] and Human IgG [Blue Line]. The working dilution of Sheep IgG MX Hu is 1:71,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.")
![Western Blot: Sheep anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72740] - Western Blot of Sheep anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Sheep anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Sheep-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72740-img0011.jpg "Western Blot: Sheep anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72740] - Western Blot of Sheep anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Sheep anti-Rabbit IgG (H+L) Secondary antibody (Pre-adsorbed) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Immunocytochemistry/Immunofluorescence: Sheep anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72740] - Immunocytochemistry/Immunofluorescence: Sheep anti-Rabbit IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72960]](http://images.novusbio.com/fullsize/Sheep-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72740-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Sheep anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72740] - Using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Sheep-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Fluorophore-linked-immunosorbent-assay-NBP1-72740-img0012.jpg)