| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33735-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33791-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97802-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5139-SP | |||
![Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human colon, kidney, liver and testis using Anti-p107 antibody NBP2-33735 (A) shows similar protein distribution across tissues to independent antibody NBP2-33791 (B).](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33735-img0014.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human colon, kidney, liver and testis using Anti-p107 antibody NBP2-33735 (A) shows similar protein distribution across tissues to independent antibody NBP2-33791 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: p107 Antibody [NBP2-33735] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/p107-Antibody-Western-Blot-NBP2-33735-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: p107 Antibody [NBP2-33735] - Selinexor alters pocket protein expression in nuclear and cytoplasmic compartments. Representative images of p107, p130 and RB (green) IF staining of bladder cancer cells treated with vehicle (V) or selinexor (S) for 48 hours. Tubulin staining (red) and DAPI staining (blue) served to define the cytoplasmic and nuclear compartments, respectively. The inserts are magnifications of the boxed cells. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/26179), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p107-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33735-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: p107 Antibody [NBP2-33735] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/p107-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33735-img0007.jpg)
![Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human testis shows moderate nuclear postivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33735-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human colon shows moderate nuclear positivity in glanular cells.](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33735-img0011.jpg)
![Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human kidney shows moderate nuclear postivity in cells in tubules.](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33735-img0012.jpg)
![Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33735-img0013.jpg)
![Western Blot: p107 Antibody [NBP2-33735] - Selinexor alters pocket protein expression in nuclear & cytoplasmic compartments(A) Representative images of p107, p130 & RB (green) IF staining of bladder cancer cells treated with vehicle (V) or selinexor (S) for 48 hours. Tubulin staining (red) & DAPI staining (blue) served to define the cytoplasmic & nuclear compartments, respectively. The inserts are magnifications of the boxed cells. (B) Quantification of staining intensity of pocket proteins normalized to DAPI. (C) Nuclear & cytoplasmic fractions of cell treated with vehicle or 0.15 uM selinexor (UM-UC-3 & T24 cells), 0.25 uM selinexor (J82) & 0.5 uM selinexor (TCCSUP) for 72 hours were assessed for the expression of RB, p107 & p130. Nup62 & tubulin were used as markers for the nuclear & cytoplasmic fractions, respectively. (D) T24 & UM-UC-3 cells transfected with siC or siRB & were treated with vehicle or 0.1 uM selinexor for 72 hours. The results are shown as percent cell viability comparing drug treated to vehicle treated cells. (E) Palbociclib reduces T24 & UM-UC-3 bladder tumor cells viability in a dose dependent manner. (F) Combined selinexor (0.1 uM) & palbociclib (0.5 uM) treatment is more effective in reducing viability of cells than either treatment alone where the CI = 1.04 for UM-UC-3 cells & 1.02 for T24 cells indicating an additive response. Error bars = ± standard deviation. Student’s t test; * denotes p ≤ 0.05, ** denotes p ≤ 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30349650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33735_rabbit-polyclonal-p107-antibody-310202416121934.jpg)
![Immunocytochemistry/ Immunofluorescence: p107 Antibody [NBP2-33735] - Selinexor alters pocket protein expression in nuclear & cytoplasmic compartments(A) Representative images of p107, p130 & RB (green) IF staining of bladder cancer cells treated with vehicle (V) or selinexor (S) for 48 hours. Tubulin staining (red) & DAPI staining (blue) served to define the cytoplasmic & nuclear compartments, respectively. The inserts are magnifications of the boxed cells. (B) Quantification of staining intensity of pocket proteins normalized to DAPI. (C) Nuclear & cytoplasmic fractions of cell treated with vehicle or 0.15 uM selinexor (UM-UC-3 & T24 cells), 0.25 uM selinexor (J82) & 0.5 uM selinexor (TCCSUP) for 72 hours were assessed for the expression of RB, p107 & p130. Nup62 & tubulin were used as markers for the nuclear & cytoplasmic fractions, respectively. (D) T24 & UM-UC-3 cells transfected with siC or siRB & were treated with vehicle or 0.1 uM selinexor for 72 hours. The results are shown as percent cell viability comparing drug treated to vehicle treated cells. (E) Palbociclib reduces T24 & UM-UC-3 bladder tumor cells viability in a dose dependent manner. (F) Combined selinexor (0.1 uM) & palbociclib (0.5 uM) treatment is more effective in reducing viability of cells than either treatment alone where the CI = 1.04 for UM-UC-3 cells & 1.02 for T24 cells indicating an additive response. Error bars = ± standard deviation. Student’s t test; * denotes p ≤ 0.05, ** denotes p ≤ 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30349650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33735_rabbit-polyclonal-p107-antibody-31020241612217.jpg)
![Western Blot: p107 Antibody [NBP2-33791] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/p107-Antibody-Western-Blot-NBP2-33791-img0004.jpg "Western Blot: p107 Antibody [NBP2-33791] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: p107 Antibody [NBP2-33791] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p107-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33791-img0005.jpg)
![Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33791] - Staining of human colon.](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33791-img0007.jpg)
![Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33791] - Staining of human kidney.](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33791-img0008.jpg)
![Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33791] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33791-img0009.jpg)
![Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33791] - Staining of human testis.](http://images.novusbio.com/fullsize/p107-Antibody-Immunohistochemistry-Paraffin-NBP2-33791-img0010.jpg)
![Western Blot: p107 Antibody [NBP2-97802] - Anti-p107 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 121 kDa. Observed band size: 121 kDa](http://images.novusbio.com/fullsize/p107-Antibody-Western-Blot-NBP2-97802-img0003.jpg "Western Blot: p107 Antibody [NBP2-97802] - Anti-p107 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 121 kDa. Observed band size: 121 kDa")
![Immunocytochemistry/Immunofluorescence: p107 Antibody [NBP2-97802] - Immunofluorescence staining of p107 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97802 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97802_rabbit-polyclonal-p107-antibody-235202511494033.png)
![Western Blot: p107 Antibody [H00005933-B01P-50ug] - Analysis of RBL1 expression in transfected 293T cell line (H00005933-T02) by RBL1 MaxPab polyclonal antibody. Lane 1: RBL1 transfected lysate(111.54 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/p107-Antibody-Western-Blot-H00005933-B01P-50ug-img0002.jpg "Western Blot: p107 Antibody [H00005933-B01P-50ug] - Analysis of RBL1 expression in transfected 293T cell line (H00005933-T02) by RBL1 MaxPab polyclonal antibody. Lane 1: RBL1 transfected lysate(111.54 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: p107 Antibody [H00005933-B01P-50ug] - Purified MaxPab antibody to RBL1 on HeLa cell. [antibody concentration 30 ug/ml]](http://images.novusbio.com/fullsize/p107-Antibody-Immunocytochemistry-Immunofluorescence-H00005933-B01P-50ug-img0001.jpg)
![Western Blot: p107 Antibody (1A5) [H00005933-M01] - Analysis of RBL1 expression in transfected 293T cell line by RBL1 monoclonal antibody (M01), clone 1A5.Lane 1: RBL1 transfected lysate (Predicted MW: 111.54 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/p107-Antibody-1A5-Western-Blot-H00005933-M01-img0002.jpg "Western Blot: p107 Antibody (1A5) [H00005933-M01] - Analysis of RBL1 expression in transfected 293T cell line by RBL1 monoclonal antibody (M01), clone 1A5.Lane 1: RBL1 transfected lysate (Predicted MW: 111.54 KDa).Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: p107 Antibody (1A5) [H00005933-M01] - Analysis of protein-protein interactions between MYBL2 and RBL1. Mahlavu cells were stained with anti-MYBL2 rabbit purified polyclonal 1:1200 and anti-RBL1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/p107-Antibody-1A5-Proximity-Ligation-Assay-H00005933-M01-img0001.jpg)
![Western Blot: p107 Overexpression Lysate (Adult Normal) [NBL1-15192] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p107.](http://images.novusbio.com/fullsize/p107-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15192-img0002.jpg "Western Blot: p107 Overexpression Lysate (Adult Normal) [NBL1-15192] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p107.")
![Immunoprecipitation:[NBP3-18375]-Detection of human RBL1 by western blot of immunoprecipitates. Samples: Whole cell lysate from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBL1 antibody used for IP at 6 µg per reaction. RBL1 was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of RBL1. For blotting immunoprecipitated RBL1, was used at 0. 4 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-18375_rabbit-p107-pab-2312025128814.jpg "Immunoprecipitation:[NBP3-18375]-Detection of human RBL1 by western blot of immunoprecipitates. Samples: Whole cell lysate from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBL1 antibody used for IP at 6 µg per reaction. RBL1 was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of RBL1. For blotting immunoprecipitated RBL1, was used at 0. 4 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Mer2 [p Ser30] Antibody [NBP1-77754] - Detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7,500 dilution. Secondary antibody was used at 1:5,000 dilution.](http://images.novusbio.com/fullsize/Mer2-[p-Ser30]-Antibody-Western-Blot-NBP1-77754-img0001.jpg "Western Blot: Mer2 [p Ser30] Antibody [NBP1-77754] - Detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7,500 dilution. Secondary antibody was used at 1:5,000 dilution.")
![Western Blot: Mer2 [p Ser30] Antibody [NBP1-77754] - Detection of phosphorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5,000 dilution.](http://images.novusbio.com/fullsize/Mer2 [p Ser30] Antibody-Western Blot-NBP1-77754-img0003.jpg)
![Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-NBP1-21374-img0039.jpg "<b>Biological Strategies Validation. </b>Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: E2F-4 Antibody [NBP1-21374] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunohistochemistry-NBP1-21374-img0037.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: E2F-4 Antibody [NBP1-21374] - Detection of human E2F4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for IP at 6 ug per reaction. E2F4 was also immunoprecipitated by another rabbit anti-E2F4 antibody. For blotting immunoprecipitated E2F4, NBP1-21374 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunoprecipitation-NBP1-21374-img0040.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: E2F-4 Antibody [NBP1-21374] - Immunoblots of indicated proteins in OVCAR-8 and PEA1 cells transfected with control luciferase (Luc) and two independent E2F-4 siRNAs. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6789141/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Knockdown-Validated-NBP1-21374-img0041.jpg)
