| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-401-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3628-SP | |||
| Carrier Free | FAB3628P-100 | |||
| Carrier Free | FAB3628P-025 | |||
| Carrier Free | FAB3628G-100 | |||
| Carrier Free | FAB3628G-025 | |||
| Carrier Free | FAB3628A-100 | |||
| Carrier Free | FAB3628A-025 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF835-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89458-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85812-25ul | |||
![Western Blot: SP1 Antibody (5I3A8) [NBP3-15837] - Western blot analysis of extracts of various cell lines, using SP1 antibody (NBP3-15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Western-Blot-NBP3-15837-img0005.jpg "Western Blot: SP1 Antibody (5I3A8) [NBP3-15837] - Western blot analysis of extracts of various cell lines, using SP1 antibody (NBP3-15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837] - Immunofluorescence analysis of NIH-3T3 cells using SP1 antibody (NBP3-15837). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Immunocytochemistry-Immunofluorescence-NBP3-15837-img0004.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517184575.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of SP1 across chromosome 14 (upper panel) and the genomic region encompassing TINF2, a representative gene enriched in SP1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-5220251726072.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517162322.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-5220251718937.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-5220251726088.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of SP1 in the representative genomic region surrounding TINF2 gene.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517202936.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517273517.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517192996.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517251978.jpg)
![Immunocytochemistry/ Immunofluorescence: SP1 Antibody (5I3A8) [SP1] - Confocal imaging of C6 cells using SP1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517213553.jpg)
![Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SP1 antibody (NB600-232) used for WB at 0.04 ug/mL. Detection by chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SP1-Antibody-Western-Blot-NB600-232-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SP1 antibody (NB600-232) used for WB at 0.04 ug/mL. Detection by chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: SP1 Antibody [NB600-232] - FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Affinity purified rabbit anti-Sp1 used at a dilution of 1:1,000 (1 ug/mL). Detection by DAB.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NB600-232-img0008.jpg)
![Western Blot: SP1 Antibody [NB600-232] - A. Whole cell lysate (50 ug for E; 10 ug for T) from HEK 293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 ug) from HEK 293T cells. A. Affinity purified rabbit anti-Sp1 antibody used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, using each antibody at 3.75 ug/mg lysate. Subsequently, was used at 0.04 ug/mL for WB. Detection by chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).](http://images.novusbio.com/fullsize/SP1-Antibody-Western-Blot-NB600-232-img0006.jpg)
![Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SP1-Antibody-Western-Blot-NB600-233-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NB600-233-img0009.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody [NB600-233] - Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 asdetected using anti-Sp1.](http://images.novusbio.com/fullsize/SP1-Antibody-Chromatin-Immunoprecipitation-NB600-233-img0014.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NB600-233-img0011.jpg)
![Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of Human SP1 on HeLa whole cell lysate using NB600-233. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibody NB600-233. NB600-233 was used at 0.04 mcg/ml for WB.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunoprecipitation-NB600-233-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SP1 antibody NB600-233 (lot NB600-233-5) used for IP at 6 ug per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody (NB600-233-4) and another rabbit anti-SP1 antibody. For blotting immunoprecipitated SP1, NB600-233 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunoprecipitation-NB600-233-img0015.jpg)
![Western Blot: SP1 Antibody (S01-9G6) [NBP3-14976] - Western blot detection of SP1 in K562, 3T3, Hela cell lysates using SP1 Rabbit mAb (1:1000 diluted). Predicted band size: 81kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/SP1-Antibody-S01-9G6-Western-Blot-NBP3-14976-img0001.jpg "Western Blot: SP1 Antibody (S01-9G6) [NBP3-14976] - Western blot detection of SP1 in K562, 3T3, Hela cell lysates using SP1 Rabbit mAb (1:1000 diluted). Predicted band size: 81kDa. Observed band size: 90kDa.")
![Immunohistochemistry-Paraffin: SP1 Antibody (S01-9G6) [NBP3-14976] - Immunohistochemistry of SP1 in paraffin-embedded Human colon cancer tissue using SP1 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/SP1-Antibody-S01-9G6-Immunohistochemistry-Paraffin-NBP3-14976-img0002.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SP1 stained by SP1 antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20460-img0018.jpg "Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SP1 stained by SP1 antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![<b>Genetic Strategies Validation. </b>Western Blot: SP1 Antibody [NBP2-20460] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/SP1-Antibody-Western-Blot-NBP2-20460-img0016.jpg)
![Immunoprecipitation: SP1 Antibody [NBP2-20460] - Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 ug of OCT1 antibody (NBP2-20460). Western blot analysis was performed using OCT1 antibody (NBP2-20460). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunoprecipitation-NBP2-20460-img0015.jpg)
![Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 stained by SP1 antibody (NBP2-20460) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20460_rabbit-polyclonal-sp1-antibody-9102024200107.jpg)
![Western Blot: SP1 Antibody [NBP2-20460] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (NBP2-20460) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20460_rabbit-polyclonal-sp1-antibody-91020242075951.jpg)
![Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 stained by SP1 antibody (NBP2-20460) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.](http://images.novusbio.com/fullsize/nbp2-20460_rabbit-polyclonal-sp1-antibody-91020242041296.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.SP1 stained by SP1 antibody (NBP2-20460) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20460_rabbit-polyclonal-sp1-antibody-91020242021961.jpg)
![Western Blot: SP1 Antibody (1A5) [H00006667-M02] - SP1 monoclonal antibody (M02), clone 1A5 Analysis of SP1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Western-Blot-H00006667-M02-img0014.jpg "Western Blot: SP1 Antibody (1A5) [H00006667-M02] - SP1 monoclonal antibody (M02), clone 1A5 Analysis of SP1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Immunocytochemistry-Immunofluorescence-H00006667-M02-img0008.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Immunohistochemistry-Paraffin-H00006667-M02-img0012.jpg)
![Western Blot: SP1 Antibody (1A5) [H00006667-M02] - SP1 monoclonal antibody (M02), clone 1A5. Analysis of SP1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Western-Blot-H00006667-M02-img0015.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Immunohistochemistry-Paraffin-H00006667-M02-img0010.jpg)
![ELISA: SP1 Antibody (1A5) [H00006667-M02] - Detection limit for recombinant GST tagged SP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-ELISA-H00006667-M02-img0016.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89458-img0009.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89458-img0006.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89458-img0007.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-89458] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89458-img0008.jpg)
![Western Blot: SP1 Antibody (4C8) [H00006667-M03] - Analysis of SP1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SP1-Antibody-4C8-Western-Blot-H00006667-M03-img0011.jpg "Western Blot: SP1 Antibody (4C8) [H00006667-M03] - Analysis of SP1 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: SP1 Antibody (4C8) [H00006667-M03] - Analysis of monoclonal antibody to SP1 on HeLa cell. Antibody concentration 20 ug/ml](http://images.novusbio.com/fullsize/SP1-Antibody-4C8-Immunocytochemistry-Immunofluorescence-H00006667-M03-img0006.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody (4C8) [H00006667-M03] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/SP1-Antibody-4C8-Immunohistochemistry-Paraffin-H00006667-M03-img0009.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody (4C8) [H00006667-M03] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/SP1-Antibody-4C8-Immunohistochemistry-Paraffin-H00006667-M03-img0007.jpg)
![Sandwich ELISA: SP1 Antibody (4C8) [H00006667-M03] - Detection limit for recombinant GST tagged SP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SP1-Antibody-4C8-Sandwich-ELISA-H00006667-M03-img0010.jpg)
![Western Blot: SP1 Antibody [NBP1-85812] - Analysis using Anti-SP1 antibody NBP1-85812 (A) shows similar pattern to independent antibody NBP1-89458 (B).](http://images.novusbio.com/fullsize/SP1-Antibody-Western-Blot-NBP1-85812-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SP1 Antibody [NBP1-85812] - Analysis using Anti-SP1 antibody NBP1-85812 (A) shows similar pattern to independent antibody NBP1-89458 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: SP1 Antibody [NBP1-85812] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85812-img0007.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-85812] - Staining of human small intestine shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SP1-Antibody-Immunohistochemistry-Paraffin-NBP1-85812-img0006.jpg)
