| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92909-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91648-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38142-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99367-50ul | |||
![Western Blot: ACTL6B Antibody [NBP2-92909] - Analysis of extracts of various cell lines, using ACTL6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Western-Blot-NBP2-92909-img0005.jpg "Western Blot: ACTL6B Antibody [NBP2-92909] - Analysis of extracts of various cell lines, using ACTL6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.")
![Immunocytochemistry/Immunofluorescence: ACTL6B Antibody [NBP2-92909] - Analysis of A-549 cells using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92909-img0001.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded mouse jejunum using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0004.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded human kidney cancer using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0002.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded human kidney using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ACTL6B Antibody [NBP1-91648] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91648-img0007.jpg "Immunocytochemistry/Immunofluorescence: ACTL6B Antibody [NBP1-91648] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP1-91648] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP1-91648-img0010.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP1-91648] - Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP1-91648-img0008.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP1-91648] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP1-91648-img0009.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP1-91648] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP1-91648-img0011.jpg)
![Western Blot: ACTL6B Antibody [NBP2-15269] - Sample (30 ug of whole cell lysate) A: SK-N-SH 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Western-Blot-NBP2-15269-img0002.jpg "Western Blot: ACTL6B Antibody [NBP2-15269] - Sample (30 ug of whole cell lysate) A: SK-N-SH 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: ACTL6B Antibody [NBP2-15269] - Paraformaldehyde-fixed HeLa, using Actin like 6B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15269-img0001.jpg)
![BAF53B antibody detects BAF53B protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BAF53B stained by BAF53B antibody (NBP2-15269) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15269_rabbit-polyclonal-actl6b-antibody-immunocytochemistry-immunofluorescence-2611202518241622.jpg)
![Western Blot: ACTL6B Antibody [NBP3-18635] - Figure 2. Western blot analysis using the antibody directed against ACTL6B Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against ACTL6B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Western-Blot-NBP3-18635-img0001.jpg "Western Blot: ACTL6B Antibody [NBP3-18635] - Figure 2. Western blot analysis using the antibody directed against ACTL6B Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against ACTL6B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.")
![ELISA: ACTL6B Antibody [NBP3-18635] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against ACTL6B . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:76,000](http://images.novusbio.com/fullsize/ACTL6B Antibody-ELISA-NBP3-18635-img0002.jpg)
![Western Blot: ACTL6B Antibody [NBP1-55478] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Western-Blot-NBP1-55478-img0003.jpg "Western Blot: ACTL6B Antibody [NBP1-55478] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.")
![Western Blot: ACTL6B Antibody [NBP2-86950] - Host: Rabbit. Target Name: ACTL6A. Sample Type: THP-1 Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Western-Blot-NBP2-86950-img0001.jpg "Western Blot: ACTL6B Antibody [NBP2-86950] - Host: Rabbit. Target Name: ACTL6A. Sample Type: THP-1 Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Simple-Western-NB100-61628-img0007.jpg "Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: BAF53A Antibody [NB100-61628] - Western blot of WT and ARID1A-/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. BAF53A antibody (NB100-61628) used for blotting. Representative of three biological replicates. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/30506#content) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-61628-img0038.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunohistochemistry-Paraffin-NB100-61628-img0035.jpg)
![Western Blot: BAF53A Antibody [NB100-61628] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-BAF53A antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-61628-img0036.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunohistochemistry-Paraffin-NB100-61628-img0034.jpg)
![Western Blot: BAF53A Antibody [NB100-61628] - BRD9 defines a distinct, non-canonical BAF complex. a Immunoprecipitation (IP)-mass spectrometry using BRD9 or IgG antibody from mouse ESCs. The plot shows the spectral count fold change in BRD9 IP relative to IgG & the corresponding AC test p values, calculated with two technical replicates using PatternLab. In orange are proteins that satisfy FC 2, AC test p < 0.05. b Immunoblotting analysis of fractions after mouse ESC nuclear lysates were subjected to a density sedimentation assay in 10–30% glycerol gradient. LMW & HMW indicate lower & higher molecular weights, respectively. Nonspecific bands are marked with an asterisk. Molecular weights from ladder are indicated. c Immunoprecipitation (IP) experiments from mouse ESC nuclear lysates using antibodies against BRG1, BRD9, & BAF47. Blots developed using chemiluminescence are marked with double asterisks; each IP was taken from a different exposure. d Depletion IP experiment from ESCs using antibodies against BRG1 or BAF155. Each lane shows the remaining proteins after each successive IP, labeled 1 through 4. e IP experiment from ESCs using antibodies against IgG or BRD9, which was incubated in increasing concentration of urea. f Schematic of the esBAF, GBAF, & PBAF complexes in mouse ESCs. Colored in orange, pink, & mustard yellow are subunits that define each individual complex. In green is the enzymatic component, BRG1. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30510198), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-61628_rabbit-polyclonal-baf53a-antibody-310202415532068.jpg)
![Western Blot: BAF53A Antibody (5U6J8) [NBP3-16659] - Western blot analysis of extracts of various cell lines, using BAF53A Rabbit mAb (NBP3-16659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Western-Blot-NBP3-16659-img0005.jpg "Western Blot: BAF53A Antibody (5U6J8) [NBP3-16659] - Western blot analysis of extracts of various cell lines, using BAF53A Rabbit mAb (NBP3-16659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded mouse kidney using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0004.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded rat brain using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0001.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded human appendix using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0002.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded human esophageal cancer using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0003.jpg)
![Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983] - Western blot detection of BAF53A in Hela (LAne 1), MCF-7 (Lane 2), HepG2 (Lane 3), U2OS (Lane 4) using NBP3-19983 (1:1000 diluted)](http://images.novusbio.com/fullsize/BAF53A-Antibody-S03-1G4-Western-Blot-NBP3-19983-img0001.jpg "Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983] - Western blot detection of BAF53A in Hela (LAne 1), MCF-7 (Lane 2), HepG2 (Lane 3), U2OS (Lane 4) using NBP3-19983 (1:1000 diluted)")
![Western Blot: BAF53A Antibody [NB100-1421] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-1421-img0002.jpg "Western Blot: BAF53A Antibody [NB100-1421] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1421-img0004.jpg)
![Flow Cytometry: BAF53A Antibody [NB100-1421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Flow-Cytometry-NB100-1421-img0005.jpg)
![Western Blot: BAF53A Antibody [NB100-1421] - Staining of Rat Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-1421-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1421-img0003.jpg)
![Immunohistochemistry: BAF53A Antibody [NBP3-38142] - Immunohistochemistry analysis of paraffin-embedded Human placenta using BAF53A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38142_rabbit-baf53a-pab-1012202411353062.jpg "Immunohistochemistry: BAF53A Antibody [NBP3-38142] - Immunohistochemistry analysis of paraffin-embedded Human placenta using BAF53A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: BAF53A Antibody [NBP3-38142] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using BAF53A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38142_rabbit-baf53a-pab-101220241136084.jpg)
![Immunohistochemistry: BAF53A Antibody [NBP3-38142] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using BAF53A Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38142_rabbit-baf53a-pab-1012202411433437.jpg)
![Western Blot: BAF53A Antibody [NBP3-38142] - Western blot analysis of various lysates using BAF53A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38142_rabbit-baf53a-pab-1012202411392957.jpg)
![Western Blot: BAF53A Antibody [NBP2-99367] - Anti-BAF53A rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47kDa](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP2-99367-img0003.jpg "Western Blot: BAF53A Antibody [NBP2-99367] - Anti-BAF53A rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47kDa")
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NBP2-99367] - Immunofluorescence staining of BAF53A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99367 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-99367_rabbit-polyclonal-baf53a-antibody-235202514331516.png)