| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14066-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94189-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4670-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1866-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47812-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32031-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08976 | |||
![Immunohistochemistry-Paraffin: OA1 Antibody [NBP2-14066] - Staining in human skin and skeletal muscle tissues . Corresponding GPR143 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/OA1-Antibody-Immunohistochemistry-Paraffin-NBP2-14066-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OA1 Antibody [NBP2-14066] - Staining in human skin and skeletal muscle tissues . Corresponding GPR143 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OA1 Antibody [NBP2-14066] - Staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/OA1-Antibody-Immunohistochemistry-Paraffin-NBP2-14066-img0004.jpg)
![Immunohistochemistry-Paraffin: OA1 Antibody [NBP2-14066] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/OA1-Antibody-Immunohistochemistry-Paraffin-NBP2-14066-img0003.jpg)
![Western Blot: OA1 Antibody [NBP2-94189] - Analysis of extracts of various cell lines, using OA1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.](http://images.novusbio.com/fullsize/OA1-Antibody-Western-Blot-NBP2-94189-img0001.jpg "Western Blot: OA1 Antibody [NBP2-94189] - Analysis of extracts of various cell lines, using OA1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.")
![Immunohistochemistry-Paraffin: OA1 Antibody [NLS2128] - Analysis of anti-GPR143 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/OA1-Antibody-Immunohistochemistry-Paraffin-NLS2128-img0002.jpg "Immunohistochemistry-Paraffin: OA1 Antibody [NLS2128] - Analysis of anti-GPR143 antibody with human brain, glioblastoma.")
![Immunohistochemistry-Paraffin: OA1 Antibody [NLS2128] - Analysis of anti-GPR143 antibody with eye, retina.](http://images.novusbio.com/fullsize/OA1-Antibody-Immunohistochemistry-Paraffin-NLS2128-img0001.jpg)
![Western Blot: OA1 Antibody [H00004935-B01P] - Analysis of GPR143 expression in transfected 293T cell line by GPR143 polyclonal antibody. Lane 1: GPR143 transfected lysate(46.64 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/OA1-Antibody-Western-Blot-H00004935-B01P-img0004.jpg "Western Blot: OA1 Antibody [H00004935-B01P] - Analysis of GPR143 expression in transfected 293T cell line by GPR143 polyclonal antibody. Lane 1: GPR143 transfected lysate(46.64 KDa). Lane 2: Non-transfected lysate.")
![Flow Cytometry: OA1 Antibody [H00004935-B01P] - Analysis of negative control 293 cells (Black) and GPR143 expressing 293 cells (Green) using GPR143 purified mouse polyclonal antibody.](http://images.novusbio.com/fullsize/OA1-Antibody-Flow-Cytometry-H00004935-B01P-img0003.jpg)
![<b>Biological Strategies Validation. </b>Increased CD47 expression in aging and replicative senescence. (A) Quantification of relative CD47 mRNA levels by qPCR. Expression levels of senescent cells were normalized to the respective proliferating control (white bar). Data are representative of three independent experiments. All values are means ± SEM. **P < 0.005, ***P < 0.0005, ****P < 0.0001. Statistically significant differences were determined by unpaired Student’s t test. (B) Representative immunofluorescence images from indicated cell types stained for CD47 (green) and Hoechst (blue). Scale bar: 20 µm, except for 3T3 cells: 50 µm. (C) Whole-cell lysates from indicated cell types were isolated and analyzed by Western blotting for the indicated proteins. Senescence was induced by chemical treatment (palbociclib, except for A549: etopsoside). Shown are representative blots of three independent experiments. GAPDH images are derived from the same blot as Fig. S3 (for Panc1 cells the same GAPDH loading control is additionally used in Fig. 8 G). (D) Representative immunofluorescence images of proliferating or senescent small epithelial cells (SAEC) stained for CD47 (green) and Hoechst (blue). scale bars indicate 20 µm. n = 2–3. (E) Western blot analysis of CD47 expression in SAEC cells. Senescence was induced by chemical treatment (palbociclib). GAPDH was used as loading control. n = 2. GAPDH images are derived from the same blot as Fig. S3. (F) Representative immunofluorescence images of primary lung fibroblast (proliferative or replicative senescent by serially passaging; normal healthy [NHLF] or IPF-derived [IPF]) showing CD47 (green) and Hoechst (blue) staining. scale bars indicate 20 µm. n = 2. (G) Whole-cell lysates from primary NHLF or IPF lung fibroblasts were isolated and analyzed by Western blotting for the indicated proteins. Senescence was induced by replicative passing. Shown are representative blots of three to four independent experiments. Source data are available for this figure: SourceData](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-4120241240512.jpg)
![<b>Biological Strategies Validation. </b>Increased CD47 expression in aging and replicative senescence. (A) Quantification of relative CD47 mRNA levels by qPCR. Expression levels of senescent cells were normalized to the respective proliferating control (white bar). Data are representative of three independent experiments. All values are means ± SEM. **P < 0.005, ***P < 0.0005, ****P < 0.0001. Statistically significant differences were determined by unpaired Student’s t test. (B) Representative immunofluorescence images from indicated cell types stained for CD47 (green) and Hoechst (blue). Scale bar: 20 µm, except for 3T3 cells: 50 µm. (C) Whole-cell lysates from indicated cell types were isolated and analyzed by Western blotting for the indicated proteins. Senescence was induced by chemical treatment (palbociclib, except for A549: etopsoside). Shown are representative blots of three independent experiments. GAPDH images are derived from the same blot as Fig. S3 (for Panc1 cells the same GAPDH loading control is additionally used in Fig. 8 G). (D) Representative immunofluorescence images of proliferating or senescent small epithelial cells (SAEC) stained for CD47 (green) and Hoechst (blue). scale bars indicate 20 µm. n = 2–3. (E) Western blot analysis of CD47 expression in SAEC cells. Senescence was induced by chemical treatment (palbociclib). GAPDH was used as loading control. n = 2. GAPDH images are derived from the same blot as Fig. S3. (F) Representative immunofluorescence images of primary lung fibroblast (proliferative or replicative senescent by serially passaging; normal healthy [NHLF] or IPF-derived [IPF]) showing CD47 (green) and Hoechst (blue) staining. scale bars indicate 20 µm. n = 2. (G) Whole-cell lysates from primary NHLF or IPF lung fibroblasts were isolated and analyzed by Western blotting for the indicated proteins. Senescence was induced by replicative passing. Shown are representative blots of three to four independent experiments. Source data are available for this figure: SourceData](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-41202412403560.jpg)
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (IAP/964 + B6H12.2) [NBP2-44409] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CD47 Antibody (IAP/964 + B6H12.2) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/CD47-Antibody-IAP-964-+-B6H12-2-Immunocytochemistry-Immunofluorescence-NBP2-44409-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD47 Antibody (IAP/964 + B6H12.2) [NBP2-44409] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CD47 Antibody (IAP/964 + B6H12.2) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Frozen: CD47 Antibody (IAP/964 + B6H12.2) [NBP2-44409] - Human frozen tonsil tissue. Antibody at 1:100. IHC-Fr image submitted from a verified customer review Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/CD47-Antibody-IAP-964-+-B6H12-2-Immunohistochemistry-Frozen-NBP2-44409-img0004.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human fallopian tube shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CD47-Antibody-Immunohistochemistry-Paraffin-NBP2-32031-img0013.jpg "Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human fallopian tube shows moderate to strong membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CD47-Antibody-Immunohistochemistry-Paraffin-NBP2-32031-img0007.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human tonsil shows moderate membranous positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/CD47-Antibody-Immunohistochemistry-Paraffin-NBP2-32031-img0009.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD47-Antibody-Immunohistochemistry-Paraffin-NBP2-32031-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (CD47/3019) [NBP3-08975] - Immunofluorescence staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot.](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Immunocytochemistry-Immunofluorescence-NBP3-08975-img0004.jpg "Immunocytochemistry/Immunofluorescence: CD47 Antibody (CD47/3019) [NBP3-08975] - Immunofluorescence staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot.")
![Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Biotin-conjugated CD47 Mouse Monoclonal Antibody (IAP/3019).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Immunohistochemistry-Paraffin-NBP3-08975-img0008.jpg)
![Flow Cytometry: CD47 Antibody (CD47/3019) [NBP3-08975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Flow-Cytometry-NBP3-08975-img0005.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD47 Mouse Monoclonal Antibody (CD47/3019).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Immunohistochemistry-Paraffin-NBP3-08975-img0003.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD47 Mouse Monoclonal Antibody (CD47/3019).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Immunohistochemistry-Paraffin-NBP3-08975-img0006.jpg)
![Protein Array: CD47 Antibody (CD47/3019) [NBP3-08975] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/3019).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Protein-Array-NBP3-08975-img0007.jpg)