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177 Results for "TRP53"
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AF1355

p53 Antibody [Unconjugated]

(1 Review)
(31 Publications)
100 μg
AF1355
Western blot shows lysates of CEM human T-lymphoblastoid cell line and MCF-7 human breast cancer cell line were mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, IP, ChIP, ICC/IF, KO
Applications: WB, Simple Western, IP, ChIP, ICC/IF, KO
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Agarose GAF1355
Alexa Fluor 350 AF1355U-100UG
Alexa Fluor 405 AF1355V-100UG
Alexa Fluor 488 AF1355G-100UG
Alexa Fluor 532 AF1355X-100UG
Alexa Fluor 594 AF1355T-100UG
Alexa Fluor 647 AF1355R-100UG
Alexa Fluor 700 AF1355N-100UG
Alexa Fluor 750 AF1355S-100UG
Allophycocyanin AF1355A
Biotin BAF1355
CoraFluor 1 AF1355CL1
DyLight 350 AF1355D
DyLight 405 AF1355E
DyLight 488 AF1355K
DyLight 550 AF1355L
DyLight 594 AF1355M
DyLight 650 AF1355W
DyLight 680 AF1355Y
DyLight 755 AF1355Z
FITC AF1355F
HRP HAF1355
Janelia Fluor 525 AF1355JF525
Janelia Fluor 549 AF1355I
Janelia Fluor 646 AF1355J
Janelia Fluor 669 AF1355JF669
PE AF1355P
PerCP AF1355C
mFluor Violet 450 SE AF1355MFV450
mFluor Violet 500 SE AF1355MFV500
mFluor Violet 610 SE AF1355MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free HAF1355
Carrier Free AF1355-SP
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AF1043

p53 [p Ser15] Antibody [Unconjugated]

(14 Publications)
100 μg
AF1043
Western blot shows lysates of CEM human T-lymphoblastoid cell line untreated or exposed to 100 J/m2UV-C for the indicated time. PVDF membrane was probed with 0.2 µg/mL Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference, duplicate lysates were probed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, IP, ICC/IF
Applications: WB, IHC, IP, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 AF1043U-100UG
Alexa Fluor 405 AF1043V-100UG
Alexa Fluor 488 AF1043G-100UG
Alexa Fluor 532 AF1043X-100UG
Alexa Fluor 594 AF1043T-100UG
Alexa Fluor 647 AF1043R-100UG
Alexa Fluor 700 AF1043N-100UG
Alexa Fluor 750 AF1043S-100UG
Allophycocyanin AF1043A
Biotin AF1043B
CoraFluor 1 AF1043CL1
DyLight 350 AF1043D
DyLight 405 AF1043E
DyLight 488 AF1043K
DyLight 550 AF1043L
DyLight 594 AF1043M
DyLight 650 AF1043W
DyLight 680 AF1043Y
DyLight 755 AF1043Z
FITC AF1043F
HRP AF1043H
Janelia Fluor 525 AF1043JF525
Janelia Fluor 549 AF1043I
Janelia Fluor 646 AF1043J
Janelia Fluor 669 AF1043JF669
PE AF1043P
PerCP AF1043C
mFluor Violet 450 SE AF1043MFV450
mFluor Violet 500 SE AF1043MFV500
mFluor Violet 610 SE AF1043MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1043-SP
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MAB1355

p53 Antibody (184721)

(12 Publications)
100 μg
MAB1355
Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IP, ICC/IF
Applications: WB, IP, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB1355U-100UG
Alexa Fluor 405 FAB1355V-100UG
Alexa Fluor 488 FAB1355G-100UG
Alexa Fluor 532 FAB1355X-100UG
Alexa Fluor 594 FAB1355T-100UG
Alexa Fluor 647 FAB1355R-100UG
Alexa Fluor 700 FAB1355N-100UG
Alexa Fluor 750 FAB1355S-100UG
Allophycocyanin FAB1355A
Biotin FAB1355B
CoraFluor 1 FAB1355CL1
DyLight 350 FAB1355D
DyLight 405 FAB1355E
DyLight 488 FAB1355K
DyLight 550 FAB1355L
DyLight 594 FAB1355M
DyLight 650 FAB1355W
DyLight 680 FAB1355Y
DyLight 755 FAB1355Z
FITC FAB1355F
HRP FAB1355H
Janelia Fluor 525 FAB1355JF525
Janelia Fluor 549 FAB1355I
Janelia Fluor 646 FAB1355J
Janelia Fluor 669 FAB1355JF669
PE FAB1355P
PerCP FAB1355C
mFluor Violet 450 SE FAB1355MFV450
mFluor Violet 500 SE FAB1355MFV500
mFluor Violet 610 SE FAB1355MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1355-SP
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DYC1043-2

Human Total p53 DuoSet IC ELISA, 2 Plate

(7 Publications)
1 Kit (for 2 Plates)
DYC1043-2
ELISA p53 [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1043E
Biotin DYC1043-5
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AF1489

p53 [p Ser46] Antibody [Unconjugated]

(1 Publication)
100 μg
AF1489
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S46) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 30 minutes. For additional reference the membrane was stripped and reprobed for total p53 using Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, IHC
Applications: WB, Simple Western, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 AF1489U-100UG
Alexa Fluor 405 AF1489V-100UG
Alexa Fluor 488 AF1489G-100UG
Alexa Fluor 532 AF1489X-100UG
Alexa Fluor 594 AF1489T-100UG
Alexa Fluor 647 AF1489R-100UG
Alexa Fluor 700 AF1489N-100UG
Alexa Fluor 750 AF1489S-100UG
Allophycocyanin AF1489A
Biotin AF1489B
CoraFluor 1 AF1489CL1
DyLight 350 AF1489D
DyLight 405 AF1489E
DyLight 488 AF1489K
DyLight 550 AF1489L
DyLight 594 AF1489M
DyLight 650 AF1489W
DyLight 680 AF1489Y
DyLight 755 AF1489Z
FITC AF1489F
HRP AF1489H
Janelia Fluor 525 AF1489JF525
Janelia Fluor 549 AF1489I
Janelia Fluor 646 AF1489J
Janelia Fluor 669 AF1489JF669
PE AF1489P
PerCP AF1489C
mFluor Violet 450 SE AF1489MFV450
mFluor Violet 500 SE AF1489MFV500
mFluor Violet 610 SE AF1489MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1489-SP
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MAB1746

p53 Antibody (197643) [Unconjugated]

(5 Publications)
100 μg
MAB1746
Balb/3T3 mouse embryonic fibroblast cell line was untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 6 hours. p53 was immunoprecipitated from 500 µg of cell lysate following incubation with 1-3 µg Rat Anti-Mouse p53 Monoclonal Antibody (Catalog # MAB1746) for 1 hour on ice. p53-antibody complexes were absorbed using goat anti-rat IgG sepharose (Invitrogen). Immunoprecipitated p53 was detected by Western blot using a 1:5000 dilution of Human/Mouse/Rat p53 Horseradish Peroxidase-conjugated Antigen Affinity-purified Polyclonal Antibody (HAF1355). View our recommended buffer recipes for immunoprecipitation.
Mu
Species Glossary
Reactivity: Mu
IP
Applications: IP
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB1746U-100UG
Alexa Fluor 405 FAB1746V-100UG
Alexa Fluor 488 FAB1746G-100UG
Alexa Fluor 532 FAB1746X-100UG
Alexa Fluor 594 FAB1746T-100UG
Alexa Fluor 647 FAB1746R-100UG
Alexa Fluor 700 FAB1746N-100UG
Alexa Fluor 750 FAB1746S-100UG
Allophycocyanin FAB1746A
Biotin FAB1746B
CoraFluor 1 FAB1746CL1
DyLight 350 FAB1746D
DyLight 405 FAB1746E
DyLight 488 FAB1746K
DyLight 550 FAB1746L
DyLight 594 FAB1746M
DyLight 650 FAB1746W
DyLight 680 FAB1746Y
DyLight 755 FAB1746Z
FITC FAB1746F
HRP FAB1746H
Janelia Fluor 525 FAB1746JF525
Janelia Fluor 549 FAB1746I
Janelia Fluor 646 FAB1746J
Janelia Fluor 669 FAB1746JF669
PE FAB1746P
PerCP FAB1746C
mFluor Violet 450 SE FAB1746MFV450
mFluor Violet 500 SE FAB1746MFV500
mFluor Violet 610 SE FAB1746MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1746-SP
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MAB13552

p53 Antibody (2589A) [Unconjugated]

100 μg
MAB13552
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with doxorubicin and trichostatin A (TSA). PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for acetylated p53 (K382) at approximately 53 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, ICC/IF
Applications: WB, IHC, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB13552U-100UG
Alexa Fluor 405 FAB13552V-100UG
Alexa Fluor 488 FAB13552G-100UG
Alexa Fluor 532 FAB13552X-100UG
Alexa Fluor 594 FAB13552T-100UG
Alexa Fluor 647 FAB13552R-100UG
Alexa Fluor 700 FAB13552N-100UG
Alexa Fluor 750 FAB13552S-100UG
Allophycocyanin FAB13552A
Biotin FAB13552B
CoraFluor 1 FAB13552CL1
DyLight 350 FAB13552D
DyLight 405 FAB13552E
DyLight 488 FAB13552K
DyLight 550 FAB13552L
DyLight 594 FAB13552M
DyLight 650 FAB13552W
DyLight 680 FAB13552Y
DyLight 755 FAB13552Z
FITC FAB13552F
HRP FAB13552H
Janelia Fluor 525 FAB13552JF525
Janelia Fluor 549 FAB13552I
Janelia Fluor 646 FAB13552J
Janelia Fluor 669 FAB13552JF669
PE FAB13552P
PerCP FAB13552C
mFluor Violet 450 SE FAB13552MFV450
mFluor Violet 500 SE FAB13552MFV500
mFluor Violet 610 SE FAB13552MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB13552-SP
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DYC1839-2

Human Phospho-p53 (S15) DuoSet IC ELISA, 2 Plate

(3 Publications)
1 Kit (for 2 Plates)
DYC1839-2
ELISA p53 [p Ser15] [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1839E
Biotin DYC1839-5
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MAB2996

p53 [p Ser392] Antibody (2185A) [Unconjugated]

100 μg
MAB2996
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1uM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-p53 (S392) Monoclonal Antibody (Catalog # MAB2996) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-p53 (S392) at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, ICC/IF
Applications: WB, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB2996U-100UG
Alexa Fluor 405 FAB2996V-100UG
Alexa Fluor 488 FAB2996G-100UG
Alexa Fluor 532 FAB2996X-100UG
Alexa Fluor 594 FAB2996T-100UG
Alexa Fluor 647 FAB2996R-100UG
Alexa Fluor 700 FAB2996N-100UG
Alexa Fluor 750 FAB2996S-100UG
Allophycocyanin FAB2996A
Biotin FAB2996B
CoraFluor 1 FAB2996CL1
DyLight 350 FAB2996D
DyLight 405 FAB2996E
DyLight 488 FAB2996K
DyLight 550 FAB2996L
DyLight 594 FAB2996M
DyLight 650 FAB2996W
DyLight 680 FAB2996Y
DyLight 755 FAB2996Z
FITC FAB2996F
HRP FAB2996H
Janelia Fluor 525 FAB2996JF525
Janelia Fluor 549 FAB2996I
Janelia Fluor 646 FAB2996J
Janelia Fluor 669 FAB2996JF669
PE FAB2996P
PerCP FAB2996C
mFluor Violet 450 SE FAB2996MFV450
mFluor Violet 500 SE FAB2996MFV500
mFluor Violet 610 SE FAB2996MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB2996-SP
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MAB1489

p53 [p Ser46] Antibody (1022G) [Unconjugated]

100 μg
MAB1489
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-p53 (S46) Monoclonal Antibody (Catalog # MAB1489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for phospho-p53 (S46) at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB1489U-100UG
Alexa Fluor 405 FAB1489V-100UG
Alexa Fluor 488 FAB1489G-100UG
Alexa Fluor 532 FAB1489X-100UG
Alexa Fluor 594 FAB1489T-100UG
Alexa Fluor 647 FAB1489R-100UG
Alexa Fluor 700 FAB1489N-100UG
Alexa Fluor 750 FAB1489S-100UG
Allophycocyanin FAB1489A
Biotin FAB1489B
CoraFluor 1 FAB1489CL1
DyLight 350 FAB1489D
DyLight 405 FAB1489E
DyLight 488 FAB1489K
DyLight 550 FAB1489L
DyLight 594 FAB1489M
DyLight 650 FAB1489W
DyLight 680 FAB1489Y
DyLight 755 FAB1489Z
FITC FAB1489F
HRP FAB1489H
Janelia Fluor 525 FAB1489JF525
Janelia Fluor 549 FAB1489I
Janelia Fluor 646 FAB1489J
Janelia Fluor 669 FAB1489JF669
PE FAB1489P
PerCP FAB1489C
mFluor Violet 450 SE FAB1489MFV450
mFluor Violet 500 SE FAB1489MFV500
mFluor Violet 610 SE FAB1489MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1489-SP
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AF3306

p53 [p Ser37] Antibody [Unconjugated]

50 μg
AF3306
Western blot shows p53 immunoprecipitated from lysates of MCF-7 human breast cancer cell line using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). MCF-7 cell line was untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.4 µg/mL Rabbit Anti-Human Phospho-p53 (S37) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3306) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S37) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference the membrane was stripped and reprobed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF3306-SP
compare
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MAB13551

p53 Antibody (184727) [Unconjugated]

100 μg
MAB13551
MCF-7 cells were stained with Mouse Anti-Human p53 Monoclonal Antibody (Catalog # MAB13551, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Hu
Species Glossary
Reactivity: Hu
CyTOF-ready, Flow
Applications: CyTOF-ready, Flow
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC13551U-100UG
Alexa Fluor 405 IC13551V-100UG
Alexa Fluor 488 IC13551G-100UG
Alexa Fluor 532 FAB13551X
Alexa Fluor 594 IC13551T-100UG
Alexa Fluor 647 IC13551R-100UG
Alexa Fluor 700 IC13551N-100UG
Alexa Fluor 750 IC13551S-100UG
Allophycocyanin IC13551A
Biotin FAB13551B
CoraFluor 1 FAB13551CL1
DyLight 350 FAB13551D
DyLight 405 FAB13551E
DyLight 488 FAB13551K
DyLight 550 FAB13551L
DyLight 594 FAB13551M
DyLight 650 FAB13551W
DyLight 680 FAB13551Y
DyLight 755 FAB13551Z
FITC FAB13551F
HRP FAB13551H
Janelia Fluor 525 FAB13551JF525
Janelia Fluor 549 FAB13551I
Janelia Fluor 646 FAB13551J
Janelia Fluor 669 FAB13551JF669
PE FAB13551P
PerCP FAB13551C
mFluor Violet 450 SE FAB13551MFV450
mFluor Violet 500 SE FAB13551MFV500
mFluor Violet 610 SE FAB13551MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC13551A
Carrier Free MAB13551-SP
Carrier Free IC13551V-100UG
Carrier Free IC13551U-100UG
Carrier Free IC13551T-100UG
Carrier Free IC13551S-100UG
Carrier Free IC13551R-100UG
Carrier Free IC13551N-100UG
Carrier Free IC13551G-100UG
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