ABCA1 Antibody
Rabbit Polyclonal anti-ABCA1, Sample Size
Price: $100.00
  • Catalog Number: NB400-105SS
  • Unit Size: 0.025 ml
Product Images
Western Blot analysis of ABCA1, using NB 400-105. Samples: 40 ug of total cell post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol. Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate  Detection of ABCA1 in prostate epithelium showing luminal and membrane staining using NB400-105.  
Gene ID 19 Reference Sequence O95477 
Species Summary Hu(+) Mu(+) Ha(+) Rt(+) Application Summary FACS, IHC, IP, WB, ICC 
Purity Affinity purified Host Rabbit 
Storage

Store at 4C. Do not freeze.
Background

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester deposition in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
Research Areas

Lipid and Metabolism,     ABC Transporters,     Golgi Apparatus Markers,     Astrocyte Markers,    

Related Diseases

Tangier Disease, Atherosclerosis, Coronary Artery Disease
Immunogen

Partial peptide sequence between residues 110-1300 of the human ABCA1 gene.
Specificity

This antibody is specific for ABCA1.
Species Reactivity

NB 400-105 reacts with human, rat, hamster and mouse ABCA1. Other species have not been tested.
Uses

NB 400-105 may be used for Western analysis where a band representing ABCA1 is seen at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal.

Dilutions

flow cytometry / FACS analysis , immunohistochemistry 1:200, immunoprecipitation See Krimbou, Western Blot 1:500-1:1000, Immunocytochemistry

Concentration

1 mg/ml
Positive Controls:

* Antigen standard for ABCA1 (NM_005502) (transient overexpression lysate)
Packaging

0.025 ml of affinity purified rabbit antisera.
Buffer

Tris/citrate, pH 7-8.
Preservative

0.02% sodium azide
Alternate Names

anti-ABC 1 antibody, anti-ABC Transporter 1 antibody, anti-ABC1 antibody, anti-ATP-binding Cassette 1 antibody, anti-ATP-binding Cassette Transporter 1 antibody, anti-CERP antibody, anti-Cholesterol Efflux Regulatory Protein antibody, anti-ABC-1 antibody
Notes

It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Post-nuclear lysates of 9-cis retinoic acid and 22R-hydroxycholesterol induced raw macrophages were used for the positive control experiments (see image). ____________________________________________________________ Blocking peptide is available or can be made for this item. Lead time is 2 days to 4 weeks. Price is $ 150/0.05 ml (or 50 ug). Please enquire availability (cat# NB 400-105PEP).
Gene Symbol

ABCA1
 
 
 

1. Murthy, S., et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J. Lipid Research. 43: 1054-1064, 2002. [WB, human]

2. Burgess, J.W., et al. Trypsin-sensitive and lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I and participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press. 277(35): 31318-31326, 2002. [WB, human/mouse]

3. Haidar, B., et al. camp induces ABC1 phosphorylation activity and promotes cholesterol efflux from fibroblasts. Journal of Lipid Research. 43(12):2087-2094,2002. [IP, human]

4. Terasaka, N., et al. T-0901317, a synthetic liver X receptor ligand, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters. 536: 6-11, 2003. [IHC-P, mouse]

5. Basso, F., et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol and plasma HDL cholestrol concentrations. Journal of Lipid Research. 44:296-302, 2003. [WB, mouse]

6. Le Lay, S., et al. Regulation of ABCA1 expression and cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res. 44(8): p. 1499-1507, 2003. [WB, mouse]

7. Khovidhunkit, W., et al. Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003. [WB, mouse]

8. Wang, N., et al. A PEST sequence in ABCA1 regulates degradation by calpain protease and stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation.111(1): 99-107, 2003. [IP/WB, human]

9. Escher, G., et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry. 278(13):11015-11019, 2003. [WB, human/hamster]

10. Feng, B., et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry. 277(45):43271-73280, 2002. [WB, mouse]

11. Fukumoto, H., et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry. 277(50):48508-48513, 2002. [WB, human/mouse]

12. Koldamova, R., et al. 22R-Hydroxycholestrol and 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression and Cholesterol Efflux in Brain Cells and Decrease Amyloid beta Secretion. Journal of Biological Chemistry. 278(15):13244-13256, 2003. [WB/IHC/IF, rat]

13. Sun, Y., et al. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. Journal of Biological Chemistry. 278(30):27688-37694, 2003. [WB, mouse]

14. Witting, S. et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry. 278(41):40121-40127, 2003. [WB, mouse]

15. Krimbou, et al. Molecular Interactions between Apolipoprotein E and the ATP-binding Cassette Transporter A1 J Biol Chem. 45: 839-848, 2004. [IP, human]

16. Gaus, K., et al. Visualizing lipid structure and raft domains in living cells with two- photon microscopy. PNAS. 100(26): 15554-15559, 2003. [ICC, mouse/human]

17. Denis, M., et al. Molecular and Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry. 279(9):7384-7394,2004. [IP, human]

18. Bortnick, A: Identification and characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878, 2003. [WB, human]

19. Wagner, B: Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 and SREBP1 Gene Expression. Molecular And Cellular Biology, Aug. 2003, pp. 5780-5789. [WB, mouse]

20. Sun, Y: Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694, 2003. [WB, mouse]

21. Field, F., et al. LXR/RXR ligand activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research. 45:905-913, 2004. [WB, human]

22. Krimbou, L., et al. Molecular interactions between apoE and ABCA1: impact on apoE lipidation. Journal of Lipid Research. 45:839-848, 2004. [IP, human]

23. Brown III, J., et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer