Acetylcholinesterase Antibody
Mouse Monoclonal anti-Acetylcholinesterase (ZR3)
Price: $330.00
  • Catalog Number: NB120-2802
  • Unit Size: 0.1 ml
Gene ID  Reference Sequence  
Species Summary Fe, GP, Rb, Rt Application Summary IHC, IP 
Purity Ascites Host Mouse 
Clone ZR3
Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Research Areas

Neuroscience,    

Isotype

IgG2b
Immunogen

Purified rat brain acetylcholinesterase
Species Reactivity

Cross-reacts with Rabbit, Rat, Cat and Guinea pig.Not yet tested in other species.
Uses

Immunohistochemistry: use at a dilution of 1/1000 Immunoprecipitation: Assay dependent. Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.This antibody cannot be used in Western blot to detect AChE.Immunohistochemical staining of Ach in rat brain results in staining of nerve fibers and terminals. This antibody reacts with both the soluble and membrane-associated AChE.
Dilutions

immunohistochemistry-Fr, immunoprecipitation
Packaging

100 microlitres Liquid
Buffer

PBS with 0.05% sodium azide
Clonality

Monoclonal
 
 
 

This product has been used in:Rakonczay Z & Brimijoin S Monoclonal antibodies to rat brain acetylcholinesterase: comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species. J Neurochem 46:280-7 (1986). General / background references:Brimijoin S et al. Death of intermediolateral spinal cord neurons follows selective, complement-mediated destruction of peripheral preganglionic sympathetic terminals by acetylcholinesterase antibodies. Neuroscience 54:201-23 (1993). Padilla S et al. Paraoxon toxicity is not potentiated by prior reduction in blood acetylcholinesterase. Toxicol Appl Pharmacol 117:110-5 (1992). J. Neural. Transm. S34:139-145 (1991).