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- Catalog Number: NB110-74733
- Unit Size: 0.1 ml
| Gene ID | 40 | Reference Sequence | Q16515 |
| Species Summary | Hu(+) | Application Summary | IHC, WB |
| Purity | Whole antisera | Host | Rabbit |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Background | ACCN1 is a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. ACCN1 may play a role in neurotransmission. In addition, a heteromeric association between ACCN1 and ACCN3 (variant 1) has been observed to co-assemble into proton-gated channels sensitive to gadolinium. FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM. Interacts with PRKCABP and ACCN3. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. |
| Immunogen | A synthetic peptide as a part of human & monkey (macaca fascicularis) amiloride-sensitive cation channel 1 (ASIC1) conjugated to immunogenic carrier protein was used as the immunogen. |
| Specificity | Appears to be specific for ASIC2. |
| Localization | Cell Membrane; multi-pass membrane protein |
| Species Reactivity | Human. |
| Uses | Immunohistochemistry and Western Blot |
| Dilutions |
immunohistochemistry Western Blot 1:300 |
| Positive Controls: | * Antigen standard for ACCN1 (NM_001094) (transient overexpression lysate) |
| Packaging | 0.1 ml of Whole Rabbit antisera |
| Buffer | Lyophilised in PBS |
| Preservative | No Preservative |
| Alternate Names | anti-Amiloride-sensitive brain sodium channel antibody, anti-acid-sensing ion channel 2 antibody, anti-ASIC2 antibody, anti-brain sodium channel 1 antibody, anti-BNaC1 antibody. anti-BNC1 antibody, anti-mammalian degenerin homolog antibody, anti-ACCN antibody, anti-BNAC1 antibody, anti-MDEG antibody |
| Notes |
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. |
| Gene Symbol | ACCN1 |
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1. Price M.P, et al. J. Biol. Chem. 271:7879-7882(1996). |
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2. Waldmann R, et al. J. Biol. Chem. 271:10433-10436(1996). |
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3. Garcia-Anoveros J, et al. Proc. Natl. Acad. Sci. U.S.A. 94:1459-1464(1997). |
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4. Babinski K, et al. J. Biol. Chem. 275:28519-28525(2000). |
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5. Hruska-Hageman A.M, et al. Biochem. J. 361:443-450(2002). |