ACP1 Antibody
Rabbit Polyclonal anti-ACP1
Price: $295.00
  • Catalog Number: NB100-56474
  • Unit Size: 0.1 mg
Product Images
Western blot analysis of ACP1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using NB100-56474 at 1 ug/ml. 
Gene ID 52 Reference Sequence  
Species Summary Hu(+) Application Summary WB 
Purity protein G purified Host Rabbit 
Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
Background

Official Gene Symbol: ACP1 Gen Bank Accession Number: NP_009030 Gene ID: 52 (Human) Gene Map Locus: 2p25 (Human) ACP1 (E.C 3.1.3.2) is a unique member of Phosphotyrosine Protein phosphotase family of proteins. It is ubiquitously expressed acid phosphotase functioning as an acid phosphotase and a protein tyrosine phosphotase. ACP1 has a cysteine-based catalytic mechanism and hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate. Reports suggest a functional role of ACP1 in TCR signaling and control of cell-cell adhesion through regulation of cytoskeletion assembly.
Isotype

IgG
Immunogen

A portion of amino acids 50-100 of human ACP1 was used as the immunogen.
Specificity

The amino acid sequence used as immunogen is 100% homologous in human, 93% in dog and orangutan, 86% in chicken and 80% homologous in mouse and rat (isoform a). It is also 100% homologous in human ACP1 isoforms c and d. An uncharacterized 45 kD band is also seen and blocked by the immunizing peptide.
Species Reactivity

Cross reacts with Human.

Uses

Western Blot
Dilutions

Western Blot 1-3 ug/ml
Positive Controls

Liver lysate
Positive Controls:

* Human Liver Protein NB820-59232 * Antigen standard for ACP1 (NM_007099) (transient overexpression lysate)
Packaging

0.1 mg protein G purified Rabbit antisera.
Buffer

PBS containing 0.2% gelatin
Preservative

0.05 % Sodium Azide
Alternate Names

anti-Acid phosphatase 1 antibody
Gene Symbol

ACP1
 
 
 

1. Wo,Y.Y., McCormack,A.L., Shabanowitz,J., Hunt,D.F., Davis,J.P., Mitchell,G.L. and Van Etten,R.L. Sequencing, cloning, and expression of human red cell-type acid phosphatase, a cytoplasmic phosphotyrosyl protein phosphatase J. Biol. Chem. 267 (15), 10856-10865 (1992)

2. Bottini et.al 277 (27) 24220-24224 (July 5, 2002)

3. Giannoni et.al JBC 278 (38) 36763-36776 (September 19, 2003)