Novus Biologicals

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (3). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (1) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1 cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1 protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.

A mixture of synthetic peptides corresponding to amino acids 341-351 (CSFPGLPRHQDQPHHQ), 91-104 (CLRTQVLEDSEDSF) and 562-574 (PRGPLPTLQVVPLC) of human ACT1 was used as immunogen, GenBank no. gi|10242290|gb|AAG15367.1|AF274303_1 .

In MCF-7, an approx. 60 kDa band is observed.

Cross reacts with Human.

Western Blot

MCF-7

0.1 mg protein G purified Rabbit antisera.

0.02% Sodium Azide

TRAF3IP2