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- Catalog Number: NB100-2140
- Unit Size: 1 ml
| Product Images | ||
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| Gene ID | 10971 | Reference Sequence | |
| Species Summary | Hu(+) Mu(+) Rt(+) | Application Summary | IHC, IP, WB |
| Purity | Immunogen affinity purified | Host | Rabbit |
| Storage | Store at 4C. Do not freeze. |
| Background | 14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4). |
| Research Areas | Signal Transduction, Neuronal Cell Markers, Growth and Development, |
| Isotype | IgG |
| Immunogen | Synthetic peptide mapping to the amino terminal domain of human 14-3-3. |
| Specificity | Broadly reactive among the 14-3-3 family. This antibody recognizes alpha, beta, zeta (delta) and theta 14-3-3. |
| Localization | Cytoplasmic |
| Species Reactivity | Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Uses | This antibody is useful for Immunohistochemistry, Immunoprecipitation and Western Blot. |
| Dilutions |
immunohistochemistry , immunoprecipitation , Western Blot 1/400 |
| Positive Controls |
HeLa and Jurkat whole cell lysates. |
| Concentration | 0.2 mg/ml |
| Positive Controls: | * Hela Whole Cell Lysate NB800-PC1 * Jurkat Whole Cell Lysate NB800-PC2 * Antigen standard for YWHAQ (NM_006826) (transient overexpression lysate) |
| Packaging | 1 ml Immunogen affinity purified Rabbit antisera. |
| Buffer | 0.2% Gelatin, PBS |
| Preservative | 0.1% Sodium Azide |
| Gene Symbol | YWHAQ |
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1. Yaffe MB. 2002. How do 14-3-3 proteins work?- - Gatekeeper phosphorylation and the molecular anvil hypothesis. FEBS Lett. 513(1):53-57. |
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2. Liu MY, Cai S, Espejo A, Bedford MT, Walker CL. 2002. 14-3-3 interacts with the tumor suppressor tuberin at Akt phosphorylation sites. Cancer Res. 62(22): 6475-6480. |
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3. Tzivion G, Shen YH, Zhu J. 2001. 14-3-3 proteins; bringing new definitions to scaffolding. Oncogene 20(44):6331-6338. |
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4. Fu H, Subramanian RR, Masters SC. 2000. 14-3-3 proteins: structure, function, and regulation. Annu Rev Pharmacol Toxicol. 40:617-647. |