Western Blot Handbook: Novus Biologicals

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This step-by-step Western blot guide is intended to serve as a starting point for understanding, performing, and troubleshooting a Western blotting protocol. Learn more about:

Find information about:

Reagents and Buffers:

Positive and negative controls
Loading Controls
Buffers and Recipes

Protocol Steps:

Sample preparation
Loading and running the gel
Protein transfer from gel to membrane
Immunoblotting
Detection
Stripping and reprobing
Troubleshooting

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