Western Blot Handbook

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Western blot protocol

HRP conjugated secondaries

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Loading control guide

This step-by-step Western blot guide is intended to serve as a starting point for understanding, performing, and troubleshooting a Western blotting protocol. Learn more about:

western blot handbook Reagents and Buffers:
  • Positive and negative controls
  • Loading Controls
  • Buffers and Recipes

Protocol Steps:

  • Sample preparation
  • Loading and running the gel
  • Protein transfer from gel to membrane
  • Immunoblotting
  • Detection
  • Stripping and reprobing
  • Troubleshooting

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