Western Blot Protocol for Leptin Antibody (NB300-611)

Western Blot Protocol

1. Block with blotto 1 HR at RT
2. Dilute primary 1:2000 - 1:4000 in blotto for 1 HR at RT
3. Wash
4. Secondary GAR-AP 1:2000 (or HRP) but dilution for secondary should be specific to detection method being used
5. Wash
6. Detect