Western Blot (NBP1-33779)

- Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Tween-20
- Blocking buffer: 1xTBS; 0.2% Tween 20; 5% nonfat dry milk

For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature.