SDS-PAGE (NBP1-99038)

1. Prepare a 1.45 ml assay buffer. The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid.

2. Add 50 ul of recombinant MDH protein with various concentrations (0.015 ug, 0.03 ug) in assay buffer.

3. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.