SDS-PAGE (NBP1-72281)

1. Prepare a 1.55 ml assay buffer. The final concentrations are 50 mM potassium phosphate, 0.072 mM dihydrofolic acid, 0.1 mM beta-nicotinamide dinucleotide phosphate and 0.003 % (w/v) bovine serum albumin.

2. Add 50 ul of recombinant DHFR protein in various concentrations (2 ug, 5 ug) in assay buffer.

3. Mix by inversion and record the decrease at A340nm for 5 minutes.