SDS-PAGE (NBP1-51026)

1. Prepare a 1 ml reaction mix into a suitable container:
The final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH.

2. Add 50ul of recombinant AKR1D1 protein solution with various concentrations (1ug, 2ug) in 750ul reaction buffer.

3. Mix by inversion and incubate at 25C for 2.5 minutes.

4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.

5. Record the decrease in A340nm for 3 minutes.