SDS-PAGE (NBP1-50895)

1. Prepare an assay buffer with the following concentrations:
200 mM Tris-HCl (pH10.0) and 10 mM NAD, 20mM L-proline, and load 150 ul into each well.

2. Add 50 ul of 1.25 ug and 2.5 ug recombinant PYCR1 protein to each well.

3. Record the increase in A340nm for 5 minutes at 25C.