SDS-PAGE (NBP1-45276)

1. Prepare a 1470 ul assay buffer. The final concentrations are 93mM Tris-Hcl (pH 8.0), 0.2mM UDPG, and 1mM beta-NAD.

2. Add 20 ul of recombinant protein UGDH with 1 ug, 2 ug and 5 ug in assay buffer

3. Mix by inversion and load 200 ul of reaction mix in to a plate well.

4. Record the decrease in A340nm for 5 minutes at 25C