SDS-PAGE (NBP1-30262)

1. Prepare 1,450ul assay buffer. The final concentrations are 81 mM Triethanolamine, 1.9 mM 2-phosphorglycerate, 0.12 mM beta- NADPH, 25 mM magnesium sulfate, 100 mM potassium chloride, 1.3 mM ADP, 4 unit pyruvate kinase, 6 unit L-lactic dehydrogenas.

2. Add 50ul of recombinant protein alpha-enolase in various concentrations(0.25 ug, 0.5 ug, and 0.1 ug) in assay buffer.

3. Mix by inversion and load 200 ul of reaction mix in to a plate well.

4. Record the decrease in A340nm for 5 minutes at 25C.