SDS-PAGE (NBC1-18342)

1. Prepare 1,450ul assay buffer. The final concentrations are 81 mM Triethanolamine, 1.9 mM 2-phosphorglycerate, 0.12 mM beta- NADPH, 25 mM magnesium sulfate, 100 mM potassium chloride, 1.3 mM ADP, 4 unit pyruvate kinase, 6 unit L-lactic dehydrogenas.

2. Add 50ul of recombinant NSE protein in various concentrations (0.25 ug, 0.5 ug, 0.1 ug) in assay buffer.

3. Mix by inversion and load 200 ul of reaction mix in to a plate well.

4. Record the decrease in A340 nm for 5 minutes at 25C.