Protocol specific for FXR2 Antibody (NB300-259)

Western Blot Procedure

1. Resolve proteins on a 7.5% SDS-PAGE.

2. Transfer protein to membrane.

3. Block the membrane for 40 minutes at RT with 3% NFDM in TBST.

4. Incubate membrane in diluted anti-FXR2, NB 300-259 (diluted in blocking solution), for 2 hours at RT.

5. Wash membrane.

6. Incubate membrane in diluted anti-mouse IgG secondary antibody (diluted in blocking solution), for 1 hour at RT.

7. Wash membrane.

8. Develope membrane.