NBP1-28570 Western blot Protocol (NBP1-28570)

Run protein on a 14% gel, using HCC38 & HT-29 cell lysate at 20 ug/well. (No sonication). Dilute NBP1-28570 to 2 ug/mL in 5% milk/TBST. 6mL/strip, incubated at RT on shaker for 1 hour. Dilute anti-mouse secondary antibody at 1:4K in 5% milk/TBST, 6mL/strip, incubate at RT on shaker for 1 hour.