MSDS (NBP1-83733)

Letter of Safety Hazard Exemption

Product Name: Human Caspase-8 ELISA Kit

Catalog Number: NBP1-83733

Our review of the manufacturing process for the requested item indicates that, apart from the components listed below, it is exempt from MSDS requirement. This product contains neither hazardous substances to health or the environment at concentrations greater than or equal to 1% nor PBT/ vPvB* substances at concentrations greater than or equal to 0.1 %. As a result, a Material Safety Data Sheet is not required for this product.

For further information refer to following regulations:
European Regulation 1907/2006 Article 31
United States 29CFR 1910.1200

Although this product is considered a general laboratory reagent, we recommend that Good Laboratory Practices (GLP) be followed to ensure the safe use and disposal of this reagent.

Kit components which require Material Safety Data Sheets (provided below):
Stop Solution
Substrate Solution
1N NaOH
1N HCl
Propidium Iodide

* PBT-substances: persistent, bioaccumulative and toxic substances vPvB-substances: very persistent, very bioaccumulative substances

Europe USA

Novus Biologicals, LTD
12 Cambridge Science Park Cambridge
CB4 0FQ
United Kingdom
Phone: (011) 44-1223-426-001
Fax: (011) 44-8719-711-635
technical@novusbio.com
www.novusbio.com

Novus Biologicals, LLC
8100 Southpark Way
Littleton, CO 80120
Phone: 1-888-506-6887
Fax: 1-303-730-1966

technical@novusbio.com
www.novusbio.com

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

1. IDENTIFICATION OF THE PREPARATION AND OF THE COMPANY/UNDERTAKING

1.1 Identification of the preparation:
Identification on the label / trade name: Stop Solution
Additional identification: 1M phosphoric acid, phosphoric acid 9%, 1M H3PO4

1.2 Use of the preparation:
The stop solution is used to terminate an enzyme reaction.

1.3 Company/undertaking identification:
Novus Biologicals, LLC 8100 Southpark Way
Littleton, CO 80120
Phone: 1-888-506-6887
Fax: 1-303-730-1966
E-Mail: technical@novusbio.com

2 HAZARDS IDENTIFICATION
2.1 Hazards description:
Causes corrosion/ irritation
2.1.1 Classification:
Regulation (EC) No 1272/2008 [CLP]
Hazard classes/Hazard categories

3.1 / category 4

Hazard statement

3.2 / Category 2 H302, H312 3.3/ Category 2 H315

4.1/ Category 4

H319 H413

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

67/548/EEC or 1999/45/EC
Hazards characteristics R-Phrases
Xi R36, R38, R53

3.3 Remark:
Full text of H- and R-phrases: see section 16.
3. COMPOSITION/INFORMATION ON INGREDIENTS

3.1 Preparation / mixture related informations Description: Mixture of mentioned below ingredients:

3.2 Ingredients:

Chemical name EC-No Index-No CAS-No Amount (%) Classification according Regulation (EC) No 1272/2008 [CLP] Classifcatio n according 67/548/EEC
Hazard class/ Hazard categories Hazard- statement
Phosphoric Acid 231-633-2 015-011-00-6 7664-38-2 99 3.2/ Category 1B H314 C; R34
Water, dest. 231-791-2 - 7732-18-5 - - - -

4. FIRST AID MEASURES

4.1 General informations:
Skin- and eyecontact causes irritation
In case of indisposition contact a doctor, show this datasheet.

4.2 In case of skin contact:
Wash affected bodyparts with plenty amount of water.

4.3 In case of eye contact:
Wash the affected eye with water for a minimum of 10 minutes. In case of indisposition contact an eye-specialist.

4.4 In case of ingestion:
Immediately wash your mouth with plenty of water. Furthermore swallow water in small amounts (dilution)

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

5. FIRE-FIGHTING MEASURES

5.1 Suitable extinguishing media:
water, dry powder extinguisher, carbondioxid

5.2 Extinguishing media which must not be used for safety reasons:
Attention with dry powder extinguisher.
It's not appropriate for in door fire because raised foam causes lack of sight.

5.3 Special exposure hazards arising from the substance or preparation itself, combustion products, resulting gases:
In case of fire, despite flue gas also dangerous, product specific gases might be produced (phosphorus oxide). Because of the very low concentration of this mixture it is very unlikely that above mentioned oxides will be produced in a hazered amount.
Further data are not known.

5.4 Special protective equipment for fire-fighters:
In case of heavy smoke a respirator shall be used.

6. ACCIDENTAL RELEASE MEASURES

6.1 Personal precautions:
In case of spilled mixture safety gloves have to be worn to prevent skin contact. In case of big amounts an additional inhalation protection is recommended.

6.2 Environmental precautions:
Mixture must not discard in sewage system/ residual waste.
Dilute residues cautiously with water and clean it up with a paper towel.

6.3 Methods for cleaning up:
Spilled mixture can be cleaned up with a paper towel an discarded in an appropriate waste.
Afterwards clean bench with water.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

7. HANDLING AND STORAGE

7.1 Handling
Advices on safe handling:
Protective measures: wear protective clothing

Precautions against fire and explosion:
Development of explosive atmosphere is not possible

7.2 Storage
Technical measures and storage conditions:
Storage in well closed containers

Packaging materials:
Acid resistant containers (e.g.: glas, polyethylen) are suitable

Requirements for storage rooms and vessels:
Prevent direct sunlight and heat. Store in well aired storage rooms.

Further information on storage conditions:
Storage temperature: -20 degrees C - 8 degrees C Storage stability: stabil Maximal storage periode: 2 years

8. EXPOSURE CONTROLS / PERSONAL PROTECTION

8.1 Exposure limit values:
8.1.1 Components with occupational exposure limits requiring monitoring:

Phosphoric acid:
maximum allowable concentration
EU: short term value: 2 mg/m3 long term (8 hs): 1 mg/m3
USA: short term value no data available long term (8 hs): 1 mg/m3

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

8.2 Personal protection equipment:
Respiratory protection: not necessary
Hand protection: disposable protective gloves
Eye protection: not necessary
Body protection: lab coat, lab shoes

9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance
Physical state: liquid
Colour: colourless
Odour: odourless

9.2 Important health, safety and environmental information

9.2.1 Safety relevant data

Value Remark_
pH (20 degrees C): pH 2, acid pH paper
Melting point ( degrees C): about 150 degrees C
Boiling point ( degrees C): no data
Ignition temperature ( degrees C): no data
Vapour pressure ( degrees C): no data
Density (g/cm3): no data
Water solubility: very well soluble
Viscosity, dynamic (mPa s): no data
Explosion limits: - mixture is not explosive

10. STABILITY AND REACTIVITY
stable under mentioned storage conditions

10.1 Conditions to avoid:
Direct sunlight, high temperature

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

10.2 Materials to avoid:
Bases (cause heat development), powdery metals
(heat developemet and dynamics of the reaction depends on the acid concentration)

10.3 Hazardous decomposition products:
No data available
Because of the low acid concentration in the mixture, no hazardous gases are expected.

11. TOXICOLOGICAL INFORMATION

11.2 Acute effects (toxicity tests)

7664-38-2 phosphoric acid Effect dose Species
Acute oral toxicity LD50 = 1530 mg/kg rat
Acute dermal toxicity LD50 = 2730 mg/kg rat
Acute inhalative toxicity LC50 = 850 mg/m3 rat

Specific symptoms in animal studies:
In case of ingestion: Somnolence, Hematuria In case of skin contact: Somnolence, Infuriation In case of inhalation: no data
In case of eye contact: no data

Irritant and corrosive effects:

Exposure time Species Evaluation
Primary irritation to the skin: 24 h rabbit strong irritation
Irritation to eyes no data rabbit strong irritation
Sensitisation
In case of skin contact: no data In case of inhalation: no data

Repeated dose toxicity (sub-acute, sub-chronic, chronic)
No data available

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

CMR effects
(carcinogenity, mutagenicity and toxicity for reproduction)
No CMR effects

12. Ecological information
12.1 Ecotoxicity:
no data

12.2 Mobility:
Known or predicted distribution to environmental compartments:
In case of shifting the pH value there might be some damaging effects for aquatic organism.
The mixture must not dispose in the sewage system without pretreatment.

13. DISPOSAL CONSIDERATIONS

13.1 Appropriate disposal / Product:
All national and local laws have to be considered. This product is only allowed to be discarded by a licensed waste management company.

13.2 Waste codes / waste designations according to EWC / AVV (Austria):
Medical waste: Waste code: 97101

In other countries different conditions might be valid. All national and local laws have to be considered.
13.3 Appropriate packaging:
Rinse container with water, dispose as the product.

14. TRANSPORT INFORMATION
Official transport designation: Stop Solution, Research reagent Because product is no dangerous good, no specific codes or labes are necessary.

15. REGULATORY INFORMATION

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

15.1 EU regulations

Labelling (Regulation (EC) No 1272/2008)

Hazard components for labelling: Phosphoric Acid Signal words: Attention
Hazard pictograms: GHS07

Hazard statements: H302, H312, H315, H319, H413

16. Precautionary statements:

OTHER INFORMATION P281, P301, P302, P305, P330, P404, P410
16.1 Relevant R- and H-phrases:

H302: Harmful if swallowed
H312: Harmful in contact with skin H315: Causes skin irritation
H319: Causes serious eye irritation
H413 May cause long lasting harmful effects to aquatic life R36: Irritating to eyes
R38: Irritating to skin
R53: May cause long-term adverse effects in the aquatic environment P281: Use personal protective equipment as required
P301: IF SWALLOWED: swallow water in small amounts P302: IF ON SKIN: wash with water
P305: IF IN EYES: wash with water P330: Flush mouth
P404: Store in a closed container P410: Protect from sunlight

Material Safety Data Sheet according to EC 1907/2006
Trade name: Stop Solution

16.2 Training instructions: no data

16.4 Data sources:
BDI Helpdesk (Germany), ECHA, GESTIS (IFA)

16.5 Further information:
The above information has been assembled to the best of our knowledge but might not be complete.
Novus Biologicals can not be held liable for any damages resulting from handling with the product

Material Safety Data Sheet according to EC 1907/2006
Trade name: Substrate Solution

1. IDENTIFICATION OF THE PREPARATION AND OF THE COMPANY/UNDERTAKING

1.1 Identification of the preparation:
Identification on the label / trade name: Substrate Solution
Additional identification: TMB

1.2 Use of the preparation:
This preparation causes a coloured reaction whose intensity depends on the concentration
of the substance which has to be determined.

1.3 Company/undertaking identification:
Novus Biologicals, LLC 8100 Southpark Way
Littleton, CO 80120
Phone: 1-888-506-6887
Fax: 1-303-730-1966
E-Mail: technical@novusbio.com

2. HAZARDS IDENTIFICATION Hazards description:
According to the regulation there are no hazardous substances. Therefore no
classification and labelling is necessary.

3. COMPOSITION/INFORMATION ON INGREDIENTS
3.1 Preparation / mixture related informations
Description: Mixture of tetramethylbenzidin TMB, hydrogen peroxide, detergent Tween 20, citrate, EDTA, preserving agent kathon CG.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Substrate Solution

3.2 Hazard Ingredients:

Chemical name EG-Nr. CAS-Nr. Amount (%)
in the preperation Classification according Regulation (EC) No 1272/2008 [CLP]
(Related to the conc. form) Classifcation according 67/548/EEC
(related to the conc. form)
Hazard class/ Hazard categories Hazard- statement
3,3',5,5'-Tetramethylbenzidin 2593646 54827-17-7 EDTA 2053583 6381-92-6 5-Chlor-2-methyl-4-isothiazolin-3-on N/ A 26172-55-4 2-Methyl-4-isothiazolin-3-on N/ A 2682-20-4 Hydrogen peroxide 231-765-0 7722-84-1 3.2/1A

4. FIRST AID MEASURES

4.1 General informations: Because of the low concentrations of the ingredients seeing a
doctor is not necessary.
4.2 In case of skin contact: Wash affected bodyparts with plenty amount of water.
4.3 In case of eye contact: Wash the affected eye with water for 10 minutes.
4.4 In case of ingestion: Immediately wash your mouth with plenty of water.
Furthermore swallow water in small amounts (dilution).

5. FIRE-FIGHTING MEASURES

5.1 Suitable extinguishing media:
water, dry powder extinguisher, carbondioxid

5.2 Extinguishing media which must not be used for safety reasons:
Attention with dry powder extinguisher.
It's not appropriate for in door fire because raised foam causes lack of sight.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Substrate Solution

5.3 Special exposure hazards arising from the substance or preparation itself, combustion products, resulting gases:
The Substrate Solution itself is not flammable.
In case of fire usual flue gas will be produced. Because of the very low concentration of this preperation it is very unlikely that gases in a hazered amount will be produced.
Further data are not known.

5.4 Special protective equipment for fire-fighters:
In case of heavy smoke a respirator shall be used.

6. ACCIDENTAL RELEASE MEASURES

6.1 Personal precautions:
In case of spilled mixture safety gloves have to be worn to prevent skin contact.

6.2 Environmental precautions:
Mixture must not discard in sewage system/ residual waste.
Dilute residues cautiously with water and clean it up with a paper towel.

6.3 Methods for cleaning up:
Spilled mixture can be cleaned up with a paper towel an discarded in an appropriate waste.
Afterwards clean bench with water.

7. HANDLING AND STORAGE

7.1 Handling
Advices on safe handling:
Protective measures: wear protective clothing

Precautions against fire and explosion:
Development of explosive atmosphere is not possible

7.2 Storage
Technical measures and storage conditions:
Storage in light-proof, well closed containers.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Substrate Solution

Packaging materials:
Containers made of polyethylen are suitable.

Requirements for storage rooms and vessels:
Prevent direct sunlight and heat. Store in well aired storage rooms.

Further information on storage conditions:
Storage temperature: -20 degrees C - 8 degrees C
Storage stability: stabil Maximal storage periode: 12 months

8. EXPOSURE CONTROLS / PERSONAL PROTECTION

8.1 Exposure limit values:
8.1.1 Components with occupational exposure limits requiring monitoring:

maximum allowable concentration

./ TMB: Austria, EU, USA: no data available
./ EDTA: Austria, EU, USA: no data available
./
./ 5-Chlor-2-methyl-4-isothiazolin-3-on:
Austria: short term value: no data available

USA: long term (8hs): short/ long term: 0.05 mg/m3
no data available

./ 2-Methyl-4-isothiazolin-3-on:
Austria: short term value: no data available

USA: long term (8hs): short/ long term: 0.05 mg/m3
no data available

./ Hydrogen peroxide:
Austria: short term value: long term (8hs): 2.8 mg/m3
1.4 mg/m3
USA: short term value:
long term (8hs): no data available
1.4 mg/m3

Material Safety Data Sheet according to EC 1907/2006
Trade name: Substrate Solution

8.2 Personal protection equipment:
Respiratory protection: not necessary
Hand protection: disposable protective gloves
Eye protection: not necessary
Body protection: lab coat, lab shoes

9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance
Physical state: liquid
Colour: yellowish or bluish
Odour: odourless

9.2 Important health, safety and environmental information

9.2.1 Safety relevant data

Value Remark_
pH (20 degrees C): about pH 3, acid pH paper
Melting point: ( degrees C): no data
Boiling point ( degrees C): no data
Ignition temperature ( degrees C): no data
Vapour pressure ( degrees C): no data
Density (g/cm3): no data
Water solubility: very well soluble
Viscosity, dynamic (mPa s): no data
Explosion limits: - mixture is not explosive

10. STABILITY AND REACTIVITY
stable under mentioned storage conditions

10.1 Conditions to avoid:
Direct sunlight, high temperature
There's no dangerous reaction but the Substrate Solution will be defective.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Substrate Solution

10.2 Materials to avoid:
Heavy metal salt
There's no dangerous reaction but the Substrate Solution will be defective.

10.3 Hazardous decomposition products:
No data available

11. TOXICOLOGICAL INFORMATION

11.2 Acute effects (toxicity tests)

Chemical name Wirkdosis Spezies
3,3',5,5'-Tetramethylbenzidin Acute oral toxicity No data
EDTA-di-Natriumsalz Acute oral toxicity LD50 = 2000 mg/kg rat
5-Chlor-2-methyl-4-isothiazolin-3-on Acute oral toxicity LD50 = 3350 mg/kg rat
2-Methyl-4-isothiazolin-3-on Acute oral toxicity LD50 = 550 mg/kg rat
Hydrogen peroxide Acute oral toxicity Akute dermal toxicity LD50 = 1232 mg/kg LD50 = 3000 mg/kg rat rabbit

Specific symptoms in animal studies:
No data available

Irritant and corrosive effects:
No data available

Sensitisation
In case of skin contact: no data In case of inhalation: no data

Repeated dose toxicity (sub-acute, sub-chronic, chronic)
No data available

CMR effects
(carcinogenity, mutagenicity and toxicity for reproduction)
No CMR effects

Material Safety Data Sheet according to EC 1907/2006
Trade name: Substrate Solution

12. Ecological information

12.1 Ecotoxicity:

Aquatic toxicity Effectdose Exposure time Species
Acute fish toxicity LC50=0.19 mg/l No data Trout
Acute daphnia toxicity EC50=0.16 mg/l No data Daphnia magna
Acute algae toxicity EC50=0.018 mg/l No data Selenastrum capricornutum
These data are only related to the preserving agents 5-Chlor-2-methyl-4- isothiazolin-3-on
and 2-Methyl-4-isothiazolin-3-on.

12.2 Mobility:
Known or predicted distribution to environmental compartments:
No data available

13. DISPOSAL CONSIDERATIONS

13.1 Appropriate disposal / Product:
All national and local laws have to be considered. This product is only allowed to be discarded by a licensed waste management company.

13.2 Waste codes / waste designations according to EWC / AVV (Austria):
Medical waste: Waste code: 97101

In other countries different conditions might be valid. All national and local laws have to be considered.

13.3 Appropriate packaging:
Rinse container with water, dispose as the product.

14. TRANSPORT INFORMATION
Official transport designation: Substrate Solution
Because product is no dangerous good, no specific codes or labes are necessary.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Substrate Solution

15. REGULATORY INFORMATION

15.1 EU regulations

Labelling (Regulation (EC) No 1272/2008)

Hazard components for labelling: -
Signal words: not applicable
Hazard pictograms: not applicable
Hazard statements: not applicable

16. OTHER INFORMATION

16.1 Training instructions: no data

16.3 Data sources:
BDI Helpdesk (Germany), ECHA, GESTIS (IFA)

16.4 Further information:
The above information has been assembled to the best of our knowledge but might not be complete.
Novus Biologicals can not be held liable for any damages resulting from handling with the product

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

1. IDENTIFICATION OF THE PREPARATION AND OF THE COMPANY/UNDERTAKING

1.1 Identification of the preparation:
Identification on the label / trade name: 1N Sodiumhydroxide solution, 1N NaOH
Additional identification: NaOH 4%

1.2 Use of the preparation:
The NaOH is used to neutralize acidified samples.

1.3 Company/undertaking identification:
Novus Biologicals, LLC 8100 Southpark Way
Littleton, CO 80120
Phone: 1-888-506-6887
Fax: 1-303-730-1966
E-Mail: technical@novusbio.com

2. HAZARDS IDENTIFICATION
2.1 Hazards description:
Causes corrosion/irritation

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

2.1.1 Classification:

Regulation (EC) No 1272/2008 [CLP]
Hazard classes/Hazard categories Hazard statement
3.1/ Category 4 H302, H312
3.2/ Category 2 H315
3.3/ Category 2 H319
4.1/ Category 3 H412

67/548/EEC or 1999/45/EC
Hazards characteristics R-Phrases
Xi R36, R38, R53

2.1.2 Remark:
Full text of H- and R-phrases: see section 16.
3. COMPOSITION/INFORMATION ON INGREDIENTS

3.1 Preparation / mixture related informations Description: Mixture of mentioned below ingredients:

3.2 Ingredients:

Chemical name EC-No Index-No CAS-No Amount (%) Classification according Regulation (EC) No 1272/2008 [CLP] Classifcatio n according 67/548/EEC
Hazard class/ Hazard categories Hazard- statement
NaOH 215-185-5 011-002-00-6 1310-73-2 solid 3.2/ Category 1 H314 C; R34
Water, dest. 231-791-2 - 7732-18-5 - - - -

4. FIRST AID MEASURES

4.1 General informations:
In case of longer exposer contact a doctor, show this datasheet. Skin- and eyecontact causes corrosion.

4.2 In case of skin contact:
Wash affected bodyparts with plenty amount of water. In case of constant skin irritation contact a doctor.

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

4.3 In case of eye contact:
Wash the affected eye with water for a minimum of 10 minutes. Contact an eye-specialist.

4.4 In case of ingestion:
Immediately wash your mouth with plenty of water. Furthermore swallow water in small amounts (dilution). Don't cause vomiting. Contact a doctor.

5. FIRE-FIGHTING MEASURES

5.1 Suitable extinguishing media:
water, dry powder extinguisher, carbondioxid

5.2 Extinguishing media which must not be used for safety reasons:
Attention with dry powder extinguisher.
It's not appropriate for in door fire because raised foam causes lack of sight.

5.3 Special exposure hazards arising from the substance or preparation itself, combustion products, resulting gases:
In case of fire, despite flue gas also dangerous, product specific gases might be produced. Because of the very low concentration of this mixture it is very unlikely that oxides will be produced in a hazardous amount.
Further data are not known.

5.4 Special protective equipment for fire-fighters:
In case of heavy smoke a respirator shall be used.

6. ACCIDENTAL RELEASE MEASURES

6.1 Personal precautions:
In case of spilled mixture safety gloves have to be worn to prevent skin contact. In case of big amounts an additional inhalation protection is recommended.

6.2 Environmental precautions:
Mixture must not discard in sewage system/ residual waste.
Dilute residues cautiously with water and clean it up with a paper towel.

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

6.3 Methods for cleaning up:
Spilled mixture can be cleaned up with a paper towel an discarded in an appropriate waste.
Afterwards clean bench with water.

7. HANDLING AND STORAGE

7.1 Handling
Advices on safe handling:
Protective measures: wear protective clothing

Precautions against fire and explosion:
Development of explosive atmosphere is not possible

7.2 Storage
Technical measures and storage conditions:
Storage in well closed containers

Packaging materials:
Base resistant containers (e.g.: polyethylen) are suitable Containers made of aluminium, tin, zinc are not suitable.

Requirements for storage rooms and vessels:
Prevent direct sunlight and heat. Store in well aired storage rooms.

Further information on storage conditions:
Storage temperature: 2 - 8 degrees C
Storage stability: stabil Maximal storage periode: 2 years

8. EXPOSURE CONTROLS / PERSONAL PROTECTION

8.1 Exposure limit values:
8.1.1 Components with occupational exposure limits requiring monitoring:

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

Sodiumhydroxide solution:
maximum allowable concentration
Austria: short term value: long term (8 hs): 2 mg/m3
4 mg/m3
USA: short term value
long term (8 hs): no data available 2 mg/m3

8.2 Personal protection equipment:
Respiratory protection: only necessary in case of fumes Hand protection: disposable protective gloves
Eye protection: not necessary
Body protection: lab coat, lab shoes

9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance
Physical state: liquid
Colour: colourless
Odour: odourless

9.2 Important health, safety and environmental information
9.2.1 Safety relevant data

Value Remark_
pH (20 degrees C): pH 10, alkaline pH paper
Melting point ( degrees C): no data
Boiling point ( degrees C): no data
Ignition temperature ( degrees C): no data
Vapour pressure ( degrees C): no data
Density (g/cm3): 1.0318 g/cm3
Water solubility: very well soluble
Viscosity, dynamic (mPa s): no data
Explosion limits: - mixture is not explosive

10. STABILITY AND REACTIVITY
stable under mentioned storage conditions

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

10.1 Conditions to avoid:
Direct sunlight, high temperature

10.2 Materials to avoid:
Metals (development of hydrogen, explosion hazard) Acids (heat development)
Sal ammonic (development of ammoniac)

10.3 Hazardous decomposition products:
No data available

11. TOXICOLOGICAL INFORMATION

11.2 Acute effects (toxicity tests)

1310-73-2/ NaOH (waterfree NaOH) Effect dose Species
Acute oral toxicity LD50 = 2000 mg/kg rat
Acute dermal toxicity no data
Acute inhalative toxicity no data

Specific symptoms in animal studies:
No data available

Irritant and corrosive effects:

Exposure time Species Evaluation
Primary irritation to the skin: no data rabbit corrosion
Irritation to eyes no data rabbit corrosion

Sensitisation
In case of skin contact: no data In case of inhalation: no data

Repeated dose toxicity (sub-acute, sub-chronic, chronic)
No data available

tech@ebioscience.com europe@ebioscience.com www.bendermedsystems.com

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

CMR effects
(carcinogenity, mutagenicity and toxicity for reproduction)
No CMR effects
12. Ecological information

12.1 Ecotoxicity:

Aquatic toxicity Effectdose Exposure time Species
Acute fish toxicity LC50=45.4 mg/l 96 h Onchorhynchus mykiss
Acute daphnia toxicity EC50=76 mg/l 24 h Daphnia magna
Acute algae toxicity No data

12.2 Mobility:
Known or predicted distribution to environmental compartments:
In case of shifting the pH value there might be some damaging effects for aquatic organism.
The mixture must not dispose in the sewage system without pretreatment.

13. DISPOSAL CONSIDERATIONS

13.1 Appropriate disposal / Product:
All national and local laws have to be considered. This product is only allowed to be discarded by a licensed waste management company.

13.2 Waste codes / waste designations according to EWC / AVV (Austria):
Medical waste: Waste code: 97101

In other countries different conditions might be valid. All national and local laws have to be considered.

13.3 Appropriate packaging:
Rinse container with water, dispose as the product.

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

14. TRANSPORT INFORMATION
Official transport designation: 1N NaOH, 1N Sodiumhydroxyde solution Because product is no dangerous good, no specific codes or labes are necessary.

15. REGULATORY INFORMATION

15.1 EU regulations

Labelling (Regulation (EC) No 1272/2008)

Hazard components for labelling: Sodiumhydroxide solution, NaOH Signal words: Attention
Hazard pictograms: GHS07

Hazard statements: H302, H312, H315, H319, H412

16. Precautionary statements:

OTHER INFORMATION P281, P301, P302, P305, P330, P404, P410
16.1 Relevant R- and H-phrases:
H302: Harmful if swallowed
H312: Harmful in contact with skin H315: Causes skin irritation
H319: Causes serious eye irritation
H412 Harmful to aquatic life with long lasting effects R36: Irritating to eyes
R38: Irritating to skin
R53: May cause long-term adverse effects in the aquatic environment

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Sodiumhydroxide solution, 1N NaOH

P281: Use personal protective equipment as required P301: IF SWALLOWED: swallow water in small amounts P302: IF ON SKIN: wash with water
P305: IF IN EYES: wash with water P330: Flush mouth
P404: Store in a closed container P410: Protect from sunlight

16.2 Training instructions: no data

16.4 Data sources:
REACH Helpdesk (Austria), BDI Helpdesk Germany), ECHA, GESTIS (IFA)

16.5 Further information:
The above information has been assembled to the best of our knowledge but might not be complete.
Novus Biologicals can not be held liable for any damages resulting from handling with the product

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

1. IDENTIFICATION OF THE PREPARATION AND OF THE COMPANY/UNDERTAKING

1.1 Identification of the preparation:
Identification on the label / trade name: 1N Hydrochloric acid, 1N HCl
Additional identification: ca. 3% HCl

1.2 Use of the preparation:
The hydrochloric acid is used to acidify the samples.

1.3 Company/undertaking identification:
Novus Biologicals, LLC 8100 Southpark Way
Littleton, CO 80120
Phone: 1-888-506-6887
Fax: 1-303-730-1966
E-Mail: technical@novusbio.com

2. HAZARDS IDENTIFICATION
2.1 Hazards description:
Causes corrosion/ irritation

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

2.1.1 Classification:

Regulation (EC) No 1272/2008 [CLP]
Hazard classes/Hazard categories Hazard statement
3.1/ category 4 H302, H312
3.2/ Category 2 H315
3.3/ Category 2 H319
4.1/ Category 4 H413

67/548/EEC or 1999/45/EC
Hazards characteristics R-Phrases
Xi R36, R38, R53

2.1.2 Remark:
Full text of H- and R-phrases: see section 16.
3. COMPOSITION/INFORMATION ON INGREDIENTS

3.1 Preparation / mixture related informations Description: Mixture of mentioned below ingredients:

3.2 Ingredients:

Chemical name EC-No Index-No CAS-No Amount (%) Classification according Regulation (EC) No 1272/2008 [CLP] Classifcatio n according 67/548/EEC
Hazard class/ Hazard categories Hazard- statement
Hydrochloric Acid Water, dest. 231595-7
231-791-2 017-002-01-X
- 7647-01-0
7732-18-5 32
- 3.2/ Category 1B
- H314
- C; R34
-

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

4. FIRST AID MEASURES

4.1 General informations:
Skin- and eyecontact causes irritation
In case of indisposition contact a doctor, show this datasheet.

4.2 In case of skin contact:
Wash affected bodyparts with plenty amount of water.

4.3 In case of eye contact:
Wash the affected eye with water for a minimum of 10 minutes. In case of indisposition contact an eye-specialist.

4.4 In case of ingestion:
Immediately wash your mouth with plenty of water. Furthermore swallow water in small amounts (dilution)

5. FIRE-FIGHTING MEASURES

5.1 Suitable extinguishing media:
water, dry powder extinguisher, carbondioxid

5.2 Extinguishing media which must not be used for safety reasons:
Attention with dry powder extinguisher.
It's not appropriate for in door fire because raised foam causes lack of sight.

5.3 Special exposure hazards arising from the substance or preparation itself, combustion products, resulting gases:
In case of fire, despite flue gas also dangerous, product specific gases might be produced. Because of the very low concentration of this mixture it is very unlikely that oxides will be produced in a hazered amount.
Further data are not known.

5.4 Special protective equipment for fire-fighters:
In case of heavy smoke a respirator shall be used.

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

6. ACCIDENTAL RELEASE MEASURES

6.1 Personal precautions:
In case of spilled mixture safety gloves have to be worn to prevent skin contact. In case of big amounts an additional inhalation protection is recommended.

6.2 Environmental precautions:
Mixture must not discard in sewage system/ residual waste.
Dilute residues cautiously with water and clean it up with a paper towel.

6.3 Methods for cleaning up:
Spilled mixture can be cleaned up with a paper towel an discarded in an appropriate waste.
Afterwards clean bench with water.

7. HANDLING AND STORAGE

7.1 Handling
Advices on safe handling:
Protective measures: wear protective clothing

Precautions against fire and explosion:
Development of explosive atmosphere is not possible

7.2 Storage
Technical measures and storage conditions:
Storage in well closed containers

Packaging materials:
Acid resistant containers (e.g.: glas, polyethylen) are suitable Containers made of metal are not suitable.

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

Requirements for storage rooms and vessels:
Prevent direct sunlight and heat. Store in well aired storage rooms.

Further information on storage conditions:
Storage temperature: 2 - 8 degrees C
Storage stability: stabil Maximal storage periode: 2 years

8. EXPOSURE CONTROLS / PERSONAL PROTECTION

8.1 Exposure limit values:
8.1.1 Components with occupational exposure limits requiring monitoring: Hydrochloric Acid:
maximum allowable concentration
EU: short term value: 15 mg/m3 long term (8 hs): 8 mg/m3
USA: short term value 7 mg/m3
long term (8 hs): no data available

8.2 Personal protection equipment:
Respiratory protection: not necessary
Hand protection: disposable protective gloves
Eye protection: not necessary
Body protection: lab coat, lab shoes

9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance
Physical state: liquid
Colour: colourless
Odour: odourless

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

9.2 Important health, safety and environmental information

9.2.1 Safety relevant data

Value Remark_
pH (20 degrees C): pH 2, acid pH paper
Melting point ( degrees C): no data
Boiling point ( degrees C): no data
Ignition temperature ( degrees C): no data
Vapour pressure ( degrees C): no data
Density (g/cm3): 1.0130 g/cm3
Water solubility: very well soluble
Viscosity, dynamic (mPa s): no data
Explosion limits: - mixture is not explosive

10. STABILITY AND REACTIVITY
stable under mentioned storage conditions

10.1 Conditions to avoid:
Direct sunlight, high temperature

10.2 Materials to avoid:
No data available

10.3 Hazardous decomposition products:
No data available
Because of the low acid concentration in the mixture, no hazardous gases are expected.

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

11. TOXICOLOGICAL INFORMATION

11.2 Acute effects (toxicity tests)

7647-01-0 HCl Effect dose Species
Acute oral toxicity LD50 = 900 mg/kg rabbit
Acute dermal toxicity no data
Acute inhalative toxicity LC50 = 3124 mg/l rat

Specific symptoms in animal studies:
No data available

Irritant and corrosive effects:
No data available

Sensitisation
In case of skin contact: no data In case of inhalation: no data

Repeated dose toxicity (sub-acute, sub-chronic, chronic)
No data available
CMR effects
(carcinogenity, mutagenicity and toxicity for reproduction)
No CMR effects

12. Ecological information

12.1 Ecotoxicity:

Aquatic toxicity (1N HCl) Effectdose Exposure time Species
Acute fish toxicity LC50=826 mg/l 96 h Leuciscus idus
Acute daphnia toxicity No data
Acute algae toxicity No data

12.2 Mobility:
Known or predicted distribution to environmental compartments:
In case of shifting the pH value there might be some damaging effects for aquatic organism.
The mixture must not dispose in the sewage system without pretreatment.

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

13. DISPOSAL CONSIDERATIONS

13.1 Appropriate disposal / Product:
All national and local laws have to be considered. This product is only allowed to be discarded by a licensed waste management company.

13.2 Waste codes / waste designations according to EWC / AVV (Austria):
Medical waste: Waste code: 97101

In other countries different conditions might be valid. All national and local laws have to be considered.

13.3 Appropriate packaging:
Rinse container with water, dispose as the product.

14. TRANSPORT INFORMATION

Official transport designation: 1N HCl, 1N Hydrochloric acid
Because product is no dangerous good, no specific codes or labes are necessary.

15. REGULATORY INFORMATION

15.1 EU regulations

Labelling (Regulation (EC) No 1272/2008)

Hazard components for labelling: Hydrochloric Acid Signal words: Attention
Hazard pictograms: GHS07

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

Hazard statements: H302, H312, H315, H319, H413 Precautionary statements: P281, P301, P302, P305, P330, P404, P410

16. OTHER INFORMATION

16.1 Relevant R- and H-phrases:

H302: Harmfull if swallowed
H312: Harmfull in contact with skin H315: Causes skin irritation
H319: Causes serious eye irritation
H413 May cause long lasting harmful effects to aquatic life

R36: Irritating to eyes
R38: Irritating to skin
R53: May cause long-term adverse effects in the aquatic environment

Material Safety Data Sheet according to EC 1907/2006
Trade name: 1N Hydrochloric acid, 1N HCl

P281: Use personal protective equipment as required P301: IF SWALLOWED: swallow water in small amounts P302: IF ON SKIN: wash with water
P305: IF IN EYES: wash with water P330: Flush mouth
P404: Store in a closed container P410: Protect from sunlight

16.2 Training instructions: no data

16.4 Data sources:
BDI Helpdesk Germany), ECHA, GESTIS (IFA)

16.5 Further information:
The above information has been assembled to the best of our knowledge but might not be complete.
Novus Biologicals can not be held liable for any damages resulting from handling with the product

Material Safety Data Sheet according to EC 1907/2006
Trade name: Propidium Iodide

1. IDENTIFICATION OF THE PREPARATION AND OF THE COMPANY/UNDERTAKING

1.1 Identification of the preparation:
Identification on the label / trade name: Propidium Iodide Additional identification: 2% Propidium Iodide solution

1.2 Use of the preparation:
Propidium Iodide solution is used to stain cells.

1.3 Company/undertaking identification:
Novus Biologicals, LLC 8100 Southpark Way
Littleton, CO 80120
Phone: 1-888-506-6887
Fax: 1-303-730-1966
E-Mail: technical@novusbio.com

2. HAZARDS IDENTIFICATION
2.1 Hazards description:
Causes irritation of eyes, skin and respiratory system.

2.1.1 Classification:

Regulation (EC) No 1272/2008 [CLP]
Hazard classes/Hazard categories Hazard statement

Causes irritation on skin Causes irritation on eyes Causes irritation of respiratory system

H315 H319
H335

Material Safety Data Sheet according to EC 1907/2006
Trade name: Propidium Iodide

67/548/EEC or 1999/45/EC
Hazards characteristics R-Phrases
Xi R36, R38, R53

2.1.2 Remark:
Full text of H- and R-phrases: see section 16.

3. COMPOSITION/INFORMATION ON INGREDIENTS

3.1 Preparation / mixture related informations Description: Mixture of mentioned below ingredients:

3.2 Ingredients:

Chemical name EC-No Index-No CAS-No Amount (%) Classification according Regulation (EC) No 1272/2008 [CLP] Classifcation according 67/548/EEC
Hazard class/ Hazard categories Hazard- statement
Propidium iodide 247-081-0 No data 25535-16-4 - 3.1/ Cat. 4 H302,H312,H Xn;Xi;R36/37/38,
- 3.2/ Cat 2 332,H315 R20/21/22
3.3/ Cat 2 HH319 -
- - -
Dest. ater 231-791-2 No data 7732-18-5 - - -
NaCl 231-598-3 No data 7647-14-5 - - - -
HEPES 230-907-9 No data 7365-45-9 - - - -
CaCl2 233-140-8 017-013-00-2 10043-52-4 - 3.3/ Cat 2 H319 Xi; R36

4. FIRST AID MEASURES

4.1 General informations:
In case of indisposition contact a doctor, show this datasheet.

4.2 In case of skin contact:
Wash affected bodyparts with plenty amount of water. Consult a doctor.

4.3 In case of eye contact:
Wash the affected eye with water for a minimum of 10 minutes. In case of indisposition contact an eye-specialist.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Propidium Iodide

4.4 In case of ingestion:
Immediately wash your mouth with plenty of water. Consult a doctor.

5. FIRE-FIGHTING MEASURES

5.1 Suitable extinguishing media:
Water, dry powder extinguisher, carbondioxid

5.2 Extinguishing media which must not be used for safety reasons:
Attention with dry powder extinguisher.
It's not appropriate for in door fire because raised foam causes lack of sight.

5.3 Special exposure hazards arising from the substance or preparation itself, combustion products, resulting gases:
In case of fire, despite flue gas also dangerous, product specific gases might be produced. Because of the very low concentration of this mixture it is very unlikely that oxides will be produced in a hazardous amount.
Further data are not known.

5.4 Special protective equipment for fire-fighters:
In case of heavy smoke a respirator shall be used.

6. ACCIDENTAL RELEASE MEASURES

6.1 Personal precautions:
In case of spilled mixture safety gloves have to be worn to prevent skin contact. In case of big amounts an additional inhalation protection is recommended.

6.2 Environmental precautions:
Mixture must not discard in sewage system/ residual waste.
Dilute residues cautiously with water and clean it up with a paper towel.

6.3 Methods for cleaning up:
Spilled mixture can be cleaned up with a paper towel an discarded in an appropriate waste.
Afterwards clean bench with water.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Propidium Iodide

7. HANDLING AND STORAGE

7.1 Handling
Advices on safe handling:
Protective measures: wear protective clothing

Precautions against fire and explosion:
Development of explosive atmosphere is not possible

7.2 Storage
Technical measures and storage conditions:
Storage in well closed containers

Packaging materials:
Store in lightproof containers

Requirements for storage rooms and vessels:
Prevent direct sunlight and heat. Store in well aired storage rooms.

Further information on storage conditions:
Storage temperature: 2 - 8 degrees C
Storage stability: stable
Maximal storage periode: see product packaging

8. EXPOSURE CONTROLS / PERSONAL PROTECTION

8.1 Exposure limit values:

8.1.1 Components with occupational exposure limits requiring monitoring:

No data available

8.2 Personal protection equipment:
Respiratory protection: not necessary
Hand protection: disposable protective gloves
Eye protection: not necessary
Body protection: lab coat, lab shoes

Material Safety Data Sheet according to EC 1907/2006
Trade name: Propidium Iodide

9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Appearance
Physical state: liquid
Colour: red
Odour: odourless
9.2 Important health, safety and environmental information

9.2.1 Safety relevant data

Value Remark_
pH (20 degrees C): no data
Melting point ( degrees C): no data
Boiling point ( degrees C): no data
Ignition temperature ( degrees C): no data
Vapour pressure ( degrees C): no data
Density (g/cm3): no data
Water solubility: no data
Viscosity, dynamic (mPa s): no data
Explosion limits: - mixture is not explosive

10. STABILITY AND REACTIVITY
stable under mentioned storage conditions

10.1 Conditions to avoid:
Direct sunlight, high temperature

10.2 Materials to avoid:
No data available

10.3 Hazardous decomposition products:
No data available
Because of the low acid concentration in the mixture, no hazardous gases are expected.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Propidium Iodide

11. TOXICOLOGICAL INFORMATION

11.2 Acute effects (toxicity tests)

25535-16-4 Propidium Iodide Effect dose Species
Acute oral toxicity LD50 = 16 mg/kg mouse
Acute dermal toxicity no data
Acute inhalative toxicity no data

Specific symptoms in animal studies:
No data available

Irritant and corrosive effects:
No data available

Sensitisation
In case of skin contact: no data In case of inhalation: no data

Repeated dose toxicity (sub-acute, sub-chronic, chronic)
No data available
CMR effects
(carcinogenity, mutagenicity and toxicity for reproduction)
No CMR effects

12. Ecological information

12.1 Ecotoxicity:
No data available

12.2 Mobility:
Known or predicted distribution to environmental compartments:
In case of shifting the pH value there might be some damaging effects for aquatic organism.
The mixture must not dispose in the sewage system without pre-treatment.

Material Safety Data Sheet according to EC 1907/2006
Trade name: Propidium Iodide

13. DISPOSAL CONSIDERATIONS

13.1 Appropriate disposal / Product:
All national and local laws have to be considered. This product is only allowed to be discarded by a licensed waste management company.

13.2 Waste codes / waste designations according to EWC / AVV (Austria):
Medical waste: Waste code: 97101

In other countries different conditions might be valid. All national and local laws have to be considered.

13.3 Appropriate packaging:
Rinse container with water, dispose as the product.

14. TRANSPORT INFORMATION

Official transport designation: Propidium Iodide
Because product is no dangerous good, no specific codes or labels are necessary.

15. REGULATORY INFORMATION

15.1 EU regulations

Labelling (Regulation (EC) No 1272/2008)

Hazard components for labelling: Propidium Iodide Signal words: Attention
Hazard pictograms: GHS07

Hazard statements: H315, H319, H335

Material Safety Data Sheet according to EC 1907/2006
Trade name: Propidium Iodide

Precautionary statements: P261, P351, P338

16. OTHER INFORMATION

16.1 Relevant R-, H- and P-phrases:

H315: Causes skin irritation
H319: Causes serious eye irritation
H335 Causes irritation to respiratory system R36: Irritating to eyes
R37: Irritation to respiratory system R38: Irritating to skin
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

16.2 Training instructions: no data

16.4 Data sources:
BDI Helpdesk Germany), ECHA, GESTIS (IFA)

16.5 Further information:
The above information has been assembled to the best of our knowledge but might not be complete.
Novus Biologicals can not be held liable for any damages resulting from handling with the product

DISPOSAL CONSIDERATIONS
Appropriate disposal / Product:
All national and local laws have to be considered. This product is only allowed to be discarded by a licensed waste management company.

Other Information
The information contained in this material safety datasheet is believed to be accurate but it is the responsibility of the user or supplier to determine the applicability of these data to the formulation of necessary safety precautions.
Novus shall not be held responsible for any damage resulting from the use of the above product or the information contained in this material safety data sheet. Copyright Novus Biologicals, LLC